FastQCFastQC Report
Fri 2 Oct 2015
OlyOv6_combined_R2_val_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOlyOv6_combined_R2_val_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193692997
Sequences flagged as poor quality0
Sequence length20-62
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTGCCAATCCCACTAGCCAATACGTTCTCTACTAGTGGATTAGCCTGTTATCCCCGGC2984790.15409901474135382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGC1276200.023.77275856
CCCGGCG838800.016.81075556
ATTAGCC1217650.016.27991143
CGAGGTG1176900.016.2714121
TCCCCGG1328850.016.0892755
TACGTTC1280850.015.25674725
TTAGCCT1305750.015.24332144
ATCCCCG1328550.015.23419654
ACTAGCC1279950.015.19240316
TAGCCTG1318050.015.109632545
ACGGTCC352750.014.92926432
CCCACTA1313750.014.92452213
CTAGCCA1327750.014.7301717
GGATTAG1371250.014.51158741
CACTAGC1362150.014.40303315
CTACTAG1398300.014.22461133
AACGGTC380100.014.11876731
ATACGTT1403550.014.04140724
TATCCCC1456200.013.8257953
GAGGTGC1390450.013.8140052
Produced by FastQC (version 0.11.2)