FastQCFastQC Report
Fri 2 Oct 2015
OlyOv6_combined_R1_val_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOlyOv6_combined_R1_val_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193692997
Sequences flagged as poor quality0
Sequence length20-62
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTGCCAATCCCACTAGCCAATACGTTCTCTACTAGTGGATTAGCCTGTTATCCCCGGC2316850.11961454651868493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGC1148750.022.50989756
CCCGGCG791400.016.00813756
ATTAGCC1152850.015.66526243
TCCCCGG1227900.015.65304355
CGAGGTG1128250.015.57241251
ACGGTCC341450.015.1330732
ATCCCCG1229750.014.95712654
TAGCCTG1242650.014.71858545
TTAGCCT1239200.014.682425544
TACGTTC1219850.014.60438725
ACTAGCC1221350.014.54791716
CTAGCCA1268400.014.19120617
CCCACTA1264000.014.14976613
GGATTAG1302750.013.955708541
CTACTAG1321500.013.69230333
AACGGTC382650.013.67796131
CACTAGC1307450.013.6134815
TATCCCC1372950.013.39046753
GTTATCC1380300.013.37600951
CCTGTTA1387150.013.28972848
Produced by FastQC (version 0.11.2)