FastQCFastQC Report
Fri 2 Oct 2015
OlyOv6_combined_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOlyOv6_combined_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200998380
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10478920.5213435053556154TruSeq Adapter, Index 9 (100% over 50bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC2835290.14106034088433947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG3653550.048.612711
GTCACGA1966200.047.82013329
CACGATC2014150.047.09152231
CACACGT3770900.046.63228612
GATCGGA3829250.046.4854431
CACGTCT3773850.046.3831614
AGTCACG2411600.046.27090528
ACACGTC3816500.045.9706113
CGGAAGA3893800.045.650394
TCGGAAG3903800.045.5325553
GAGCACA3947550.045.2545289
AGAGCAC3963950.045.122018
AGCACAC3980950.044.83265710
ATCGGAA4046750.043.998152
ACGTCTG3981250.043.93655815
CGTCTGA3992150.043.76119216
TCACGAT2159850.043.3959830
CGATCAG2177850.043.1097333
GAACTCC4095400.042.5459321
ACGATCA2226550.042.393932
Produced by FastQC (version 0.11.2)