FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 20150521_trimmed_2212_lane2_400ppm_GCCAAT.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 79218039 |
| Filtered Sequences | 0 |
| Sequence length | 1-51 |
| %GC | 33 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
![[FAIL]](Icons/error.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGGGG | 253495 | 1416.3623 | 1541.9165 | 8 |
| GGTGG | 683280 | 617.4341 | 674.45416 | 10 |
| GGGTG | 487960 | 440.93655 | 480.45013 | 10 |
| GTGGG | 412555 | 372.79816 | 397.72357 | 13 |
| GGCGG | 545895 | 348.8133 | 382.12082 | 5 |
| TGGGG | 374890 | 338.76282 | 367.82526 | 3 |
| GGGGT | 328225 | 296.59482 | 321.87463 | 21 |
| GGAGG | 524815 | 227.84459 | 244.47081 | 10 |
| GGGCG | 355955 | 227.4464 | 252.90916 | 19 |
| GCGGG | 317240 | 202.70845 | 216.65598 | 5 |
| GAGGG | 396555 | 172.16145 | 186.08427 | 4 |
| TGGTG | 1127620 | 164.7941 | 175.55803 | 6 |
| GGGAG | 350975 | 152.37323 | 162.48395 | 2 |
| CGGGG | 229435 | 146.60324 | 163.00172 | 7 |
| GGGGC | 222015 | 141.86205 | 155.59569 | 3 |
| GGGGA | 299220 | 129.90417 | 144.0037 | 7 |
| GGTTG | 864860 | 126.393486 | 136.61357 | 18 |
| GTGGT | 850860 | 124.34748 | 134.84694 | 2 |
| GTTGG | 825065 | 120.57771 | 129.94205 | 1 |
| AGGGG | 261905 | 113.70414 | 122.376526 | 2 |
| GTGTG | 777635 | 113.64613 | 132.4384 | 1 |
| GGTGT | 764335 | 111.70243 | 120.77778 | 1 |
| TGTGG | 740365 | 108.19938 | 116.05247 | 6 |
| TGGGT | 601500 | 87.90519 | 96.28599 | 8 |
| TTGGG | 599995 | 87.68524 | 93.45228 | 7 |
| GGGTT | 578420 | 84.5322 | 93.91241 | 6 |
| TGGCG | 584965 | 60.45062 | 65.18484 | 10 |
| GCTGG | 570245 | 58.929443 | 66.82289 | 1 |
| GCGGC | 702540 | 51.337406 | 56.43608 | 3 |
| CGGCG | 697750 | 50.98738 | 57.017048 | 4 |
| GATGG | 702925 | 49.35469 | 53.04487 | 17 |
| GGTGA | 679885 | 47.736973 | 51.66913 | 7 |
| GGTCG | 416590 | 43.050648 | 49.155327 | 13 |
| GCGGT | 401180 | 41.45817 | 45.256603 | 8 |
| GCGCG | 564310 | 41.23639 | 44.115322 | 6 |
| GGATG | 579520 | 40.690014 | 44.956314 | 9 |
| GGCGC | 546305 | 39.92069 | 43.412567 | 7 |
| GGCTG | 385735 | 39.862072 | 43.349453 | 15 |
| TGTTG | 1685530 | 39.838436 | 41.90158 | 45 |
| GGCGT | 381715 | 39.446648 | 43.01384 | 8 |
| GTCGG | 378035 | 39.066353 | 42.835297 | 3 |
| GTGGC | 372065 | 38.44941 | 44.39741 | 5 |
| TGAGG | 539370 | 37.870953 | 40.943024 | 30 |
| CGGTG | 362865 | 37.498676 | 41.813107 | 10 |
| TGCGG | 361870 | 37.39585 | 40.378654 | 12 |
| GGTGC | 345330 | 35.6866 | 39.22503 | 7 |
| AGGTG | 498555 | 35.005196 | 38.33422 | 9 |
| GAGGT | 493770 | 34.66922 | 37.448425 | 44 |
| CGCGG | 473835 | 34.62502 | 39.039715 | 1 |
| GCGTG | 334750 | 34.593254 | 37.816444 | 3 |
| TGGAG | 481355 | 33.797527 | 36.10128 | 15 |
| GTGGA | 464930 | 32.64427 | 35.646576 | 7 |
| AGGGT | 444270 | 31.193663 | 36.38778 | 2 |
| GGCCG | 417625 | 30.517529 | 34.654522 | 1 |
| TTGGT | 1288625 | 30.457367 | 32.533005 | 23 |
| TCGGG | 293645 | 30.34544 | 33.991066 | 6 |
| GTGAG | 423495 | 29.734985 | 31.550375 | 23 |
| GTTGT | 1227590 | 29.01477 | 30.641567 | 10 |
| AGTGG | 411565 | 28.897339 | 31.414701 | 4 |
| GTTTG | 1215190 | 28.72169 | 30.647165 | 47 |
| CGTGG | 272970 | 28.208874 | 32.828804 | 4 |
| TGGTT | 1180895 | 27.911112 | 30.168055 | 5 |
| TGGGC | 269420 | 27.842018 | 30.358974 | 15 |
| GAGTG | 395180 | 27.746895 | 30.755587 | 2 |
| GGGAT | 392925 | 27.588562 | 30.363998 | 8 |
| GCCGG | 369360 | 26.990612 | 29.347473 | 8 |
| ATGGG | 381740 | 26.803228 | 29.067886 | 7 |
| GGCGA | 536735 | 26.648413 | 30.697006 | 20 |
| CGGGC | 364480 | 26.63401 | 29.703875 | 6 |
| TGGGA | 379235 | 26.627344 | 29.002874 | 12 |
| TTTGG | 1091175 | 25.790524 | 27.402151 | 15 |
| GTAGG | 366570 | 25.738092 | 27.862999 | 22 |
| GGTAG | 362770 | 25.471281 | 27.143288 | 1 |
| TTGTG | 1077155 | 25.459156 | 26.997576 | 8 |
| GTGTT | 1073565 | 25.374308 | 27.008377 | 8 |
| GTGCG | 240095 | 24.811556 | 26.864225 | 17 |
| TGTGT | 1045650 | 24.714518 | 27.817392 | 16 |
| CTGGG | 236595 | 24.449862 | 27.089188 | 22 |
| GGAGT | 330480 | 23.204092 | 25.598011 | 2 |
| GGGTC | 222990 | 23.043915 | 25.70267 | 19 |
| GGTTT | 930075 | 21.982841 | 23.586496 | 13 |
| CGGGT | 210045 | 21.706171 | 23.417225 | 7 |
| GGGCT | 208230 | 21.518608 | 24.106539 | 10 |
| GGGTA | 303130 | 21.28376 | 23.378239 | 3 |
| GCAGG | 417190 | 20.71311 | 22.72642 | 22 |
| AGCGG | 416140 | 20.66098 | 22.530937 | 2 |
| TAGGG | 292460 | 20.534584 | 25.454723 | 1 |
| GACGG | 403105 | 20.013803 | 22.351942 | 2 |
| GAGGC | 390345 | 19.380281 | 22.208187 | 8 |
| GGCAG | 383595 | 19.045149 | 20.681067 | 20 |
| GAGCG | 378920 | 18.81304 | 22.580446 | 7 |
| AGGCG | 378645 | 18.799387 | 20.623486 | 19 |
| GAAGG | 547030 | 18.453178 | 19.60973 | 13 |
| GCGGA | 360755 | 17.911163 | 19.963484 | 1 |
| CGAGG | 352665 | 17.509502 | 20.282785 | 7 |
| GCGAG | 345220 | 17.139866 | 19.491909 | 9 |
| CCGGG | 232950 | 17.022589 | 18.912182 | 16 |
| GGAAG | 496015 | 16.73227 | 18.416067 | 6 |
| GAGGA | 481335 | 16.237062 | 18.064157 | 4 |
| GGGCC | 216890 | 15.849022 | 17.908102 | 7 |
| GGGCA | 312210 | 15.500948 | 16.694817 | 7 |
| GGACG | 305595 | 15.172519 | 16.574425 | 25 |
| AGGAG | 441900 | 14.906786 | 15.93894 | 11 |
| GAGAG | 404415 | 13.64229 | 15.62617 | 1 |
| TGATG | 1164455 | 13.222971 | 14.272116 | 3 |
| CAGGG | 266260 | 13.21957 | 14.476181 | 2 |
| GGAGA | 391225 | 13.197347 | 14.589337 | 46 |
| GGAGC | 255780 | 12.699248 | 14.444671 | 6 |
| CGGGA | 254750 | 12.648109 | 13.95686 | 22 |
| AGAGG | 356785 | 12.035568 | 12.740637 | 3 |
| CGGAG | 238835 | 11.8579445 | 13.0545 | 26 |
| AGGGC | 237115 | 11.772548 | 12.942605 | 6 |
| ACGGG | 231115 | 11.474653 | 12.732753 | 39 |
| GGGAA | 327100 | 11.034193 | 11.847578 | 42 |
| TGCTG | 657280 | 10.985207 | 11.991873 | 20 |
| GTTGA | 961695 | 10.920529 | 11.74726 | 19 |
| AGGGA | 305635 | 10.310105 | 11.209018 | 3 |
| AAGGG | 303675 | 10.243988 | 10.978559 | 5 |
| GATTG | 846530 | 9.612773 | 10.283228 | 12 |
| GTGAT | 820970 | 9.322528 | 10.005643 | 8 |
| ATGGT | 779205 | 8.848264 | 9.683968 | 8 |
| GATGT | 756170 | 8.58669 | 9.092771 | 14 |
| GGGAC | 170050 | 8.442831 | 9.402752 | 8 |
| TTGAG | 717390 | 8.146324 | 8.643519 | 20 |
| GGTAT | 710710 | 8.07047 | 10.177829 | 46 |
| GTTGC | 473260 | 7.9096565 | 11.092742 | 47 |
| ATTGG | 686140 | 7.7914643 | 8.25674 | 10 |
| GCTTG | 455570 | 7.6140013 | 8.57609 | 21 |
| TGGAT | 669090 | 7.5978537 | 8.157048 | 2 |
| TTGGC | 450660 | 7.5319395 | 8.437819 | 3 |
| GTATG | 662775 | 7.526143 | 9.504876 | 47 |
| TTGTT | 1939155 | 7.4125094 | 7.657276 | 23 |
| TTGGA | 648130 | 7.3598413 | 7.879438 | 13 |
| TGGTA | 630290 | 7.1572604 | 7.606548 | 24 |
| CTGGT | 419225 | 7.006562 | 7.83679 | 2 |
| TTGCG | 415945 | 6.951743 | 7.571284 | 8 |
| TGGTC | 410885 | 6.8671756 | 7.59215 | 3 |
| GCGTT | 403560 | 6.744751 | 7.5166907 | 2 |
| TGTGA | 593905 | 6.7440896 | 7.141499 | 28 |
| GGTTA | 588150 | 6.678739 | 7.6413965 | 1 |
| GGATT | 583595 | 6.627014 | 7.2999673 | 12 |
| AGTTG | 578160 | 6.565297 | 7.0185523 | 18 |
| GCTGC | 553975 | 6.5469646 | 7.2223625 | 4 |
| TATGG | 571960 | 6.494893 | 7.016927 | 10 |
| TTTTG | 1694090 | 6.475737 | 6.810193 | 3 |
| TGTTT | 1689645 | 6.458746 | 6.6706476 | 35 |
| CTTGG | 384565 | 6.427285 | 7.147933 | 11 |
| TGAGT | 563080 | 6.3940563 | 6.863329 | 12 |
| ATGTG | 562040 | 6.3822465 | 6.9137983 | 15 |
| TGCCG | 539410 | 6.374833 | 7.0156674 | 7 |
| TCGGC | 530030 | 6.263979 | 6.9656687 | 15 |
| CGCCG | 746185 | 6.235746 | 7.077484 | 7 |
| TTTGT | 1622340 | 6.2014685 | 6.4482036 | 31 |
| CGTCG | 523245 | 6.1837926 | 7.097426 | 1 |
| AGGTT | 543635 | 6.1732483 | 6.5409694 | 20 |
| TCTGG | 367965 | 6.149847 | 6.6262975 | 1 |
| CGCTG | 510450 | 6.0325794 | 6.476081 | 6 |
| CGTTG | 358875 | 5.997925 | 6.545746 | 7 |
| TGTAG | 526745 | 5.9814544 | 6.3925977 | 21 |
| GGCTT | 354035 | 5.917033 | 6.513197 | 10 |
| GCCGC | 705155 | 5.892864 | 6.5112543 | 20 |
| TGTCG | 350635 | 5.8602085 | 6.345672 | 12 |
| GTTTT | 1521140 | 5.8146276 | 6.1607866 | 22 |
| GTCGT | 347590 | 5.809317 | 6.5036697 | 2 |
| TGGCT | 341370 | 5.7053614 | 6.3278832 | 18 |
| TCGCG | 482185 | 5.6985393 | 6.473393 | 6 |
| TGCGC | 474440 | 5.6070075 | 6.2226295 | 5 |
| AGTGT | 492650 | 5.594288 | 6.0205564 | 2 |
| TAGGT | 481965 | 5.4729548 | 6.640274 | 44 |
| GCTGT | 326750 | 5.4610157 | 6.0196447 | 5 |
| TTCGG | 325035 | 5.4323525 | 6.096955 | 2 |
| GTTCG | 321435 | 5.372185 | 6.1494703 | 4 |
| GTGCT | 320135 | 5.350458 | 6.046623 | 7 |
| GCGCT | 450130 | 5.319708 | 5.785032 | 11 |
| GAGTT | 468065 | 5.315113 | 5.6928687 | 17 |
| CGCGC | 632775 | 5.287997 | 5.7505064 | 10 |
| CTGGC | 446490 | 5.27669 | 5.706173 | 10 |
| TCGGT | 315000 | 5.264636 | 6.080147 | 12 |
| CGGTT | 310245 | 5.1851654 | 5.7774487 | 28 |
| CTGCG | 430255 | 5.084822 | 5.5601344 | 5 |
| GTCGC | 427865 | 5.0565767 | 5.730712 | 11 |
| TCGTG | 302060 | 5.0483685 | 5.5950103 | 3 |
| GTGTA | 442870 | 5.0290117 | 5.3965874 | 5 |
| GTAGT | 441475 | 5.0131702 | 5.466884 | 13 |
| TGCGT | 298215 | 4.9841065 | 5.4940305 | 9 |
| GCGCC | 595855 | 4.979462 | 5.402644 | 3 |
| TAGTG | 430090 | 4.883888 | 5.2745943 | 14 |
| GCTCG | 412510 | 4.8751087 | 5.4567704 | 16 |
| TTAGG | 426780 | 4.846301 | 5.350954 | 44 |
| GCCGT | 409305 | 4.8372316 | 5.5184827 | 8 |
| GTTAG | 423040 | 4.803832 | 5.454319 | 23 |
| GCGTC | 396860 | 4.6901546 | 5.2782006 | 9 |
| TGGCC | 395435 | 4.6733136 | 5.2045217 | 6 |
| GATGA | 842950 | 4.5988445 | 4.99433 | 4 |
| CCGCG | 533235 | 4.4561577 | 4.915184 | 7 |
| GCCTG | 371485 | 4.390269 | 4.822549 | 2 |
| CGCGT | 364295 | 4.3052964 | 4.845723 | 19 |
| GTCTG | 254580 | 4.254829 | 4.6347437 | 14 |
| TGTGC | 254015 | 4.245386 | 4.9834113 | 12 |
| GGTCT | 250540 | 4.187308 | 4.6897507 | 11 |
| GCCCG | 487025 | 4.0699883 | 5.4498568 | 47 |
| CGGCT | 344345 | 4.069524 | 4.470799 | 14 |
| CGGTC | 343535 | 4.0599513 | 4.6148214 | 18 |
| GCTGA | 495405 | 3.977945 | 4.4266224 | 19 |
| GATCG | 494095 | 3.9674265 | 4.4555073 | 7 |
| GGTTC | 235420 | 3.9346056 | 4.432215 | 3 |
| CGATG | 488055 | 3.9189265 | 4.2231092 | 25 |
| GCGAT | 486540 | 3.906762 | 4.226726 | 18 |
| GGCCT | 329960 | 3.8995197 | 4.3089523 | 8 |
| GATGC | 483430 | 3.8817897 | 4.3392696 | 10 |
| GTGCC | 327830 | 3.8743472 | 4.4061007 | 6 |
| TGACG | 477040 | 3.83048 | 4.1432676 | 16 |
| CTCGG | 318250 | 3.7611291 | 4.268455 | 8 |
| ATGGC | 466635 | 3.746931 | 4.305207 | 18 |
| CGTGC | 309930 | 3.662802 | 4.1813807 | 7 |
| GTGTC | 217910 | 3.6419587 | 4.014424 | 21 |
| CGGCC | 435530 | 3.6396525 | 3.9273338 | 6 |
| CTGTG | 214955 | 3.592571 | 3.9057584 | 26 |
| CGACG | 621005 | 3.526026 | 4.9520507 | 3 |
| GGCAT | 433625 | 3.4818711 | 3.8276358 | 7 |
| CGGCA | 602440 | 3.4206152 | 3.7829003 | 18 |
| TTTTT | 5489865 | 3.3939178 | 3.493464 | 16 |
| GTCGA | 418415 | 3.3597398 | 3.81578 | 2 |
| TGAAG | 615475 | 3.3578198 | 3.6227753 | 12 |
| CCGGC | 401715 | 3.3570662 | 3.6497972 | 6 |
| TGGCA | 416060 | 3.34083 | 3.7944381 | 4 |
| GGTCA | 415975 | 3.3401475 | 3.6980772 | 4 |
| CGTGT | 198755 | 3.3218184 | 3.9053867 | 4 |
| GCAGC | 584570 | 3.3191504 | 3.5559733 | 12 |
| GCCGA | 576770 | 3.2748625 | 3.7979605 | 8 |
| CCGGT | 273495 | 3.2322075 | 3.5629873 | 2 |
| GGCTC | 272195 | 3.2168438 | 3.561498 | 18 |
| CAGCG | 560335 | 3.1815457 | 3.4277189 | 13 |
| CGCGA | 555735 | 3.1554272 | 3.513892 | 8 |
| CATGG | 390675 | 3.1369965 | 3.377696 | 31 |
| AGCTG | 390415 | 3.134909 | 3.4428253 | 9 |
| GTCCG | 261685 | 3.092635 | 3.5698001 | 7 |
| TGGAA | 566070 | 3.088283 | 3.3082433 | 14 |
| ATCGG | 384050 | 3.0837998 | 3.460665 | 5 |
| TGCAG | 383175 | 3.0767734 | 3.242909 | 30 |
| GTCAG | 379135 | 3.0443335 | 3.3747177 | 20 |
| TCCGG | 257225 | 3.039926 | 3.3197763 | 12 |
| TCAGG | 375115 | 3.0120547 | 3.292437 | 18 |