FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-larvae-day5-RNA-EPI-99-5_S40_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-5_S40_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43415
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3610.8315098468271335Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3150.7255556835195209Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC470.10825751468386503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATC200.0065683555148.40417145
CGGAGTT200.0065683555148.40417145
CTCGCCG151.3996295E-4140.140381
TCGCCGC100.0079349205139.493072
TTATCGT100.007962373139.332186
TCCGTCA100.007962373139.332187
ATCCCAT403.475159E-4111.30313145
GGCATGC204.4803595E-4104.6198042
AATCCTA250.00108761883.695842
GATCGGA555.4402335E-876.44021
TCCTAAC300.002242462769.746544
GAGCGTC601.034914E-769.666099
GACGACG300.002252767369.666098
GCCACAC300.002252767369.666097
AGAGCGT601.034914E-769.666098
CGGAAGA702.982997E-759.782744
ATGCCAC350.00413082759.782745
GAGACGA350.00414978159.7137876
ACGACGA350.00414978159.7137879
CTTCTAC750.00421576259.36167145
Produced by FastQC (version 0.11.5)