FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-larvae-day5-RNA-EPI-99-5_S40_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-5_S40_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43415
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGTATCTCGTATGC1780.4099965449729356TruSeq Adapter, Index 21 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGTATCTCGTATG1730.3984797880916734TruSeq Adapter, Index 21 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGTTCCGTATCTCGTATG1680.38696303121041115TruSeq Adapter, Index 21 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCAAGTTCCGTATCTCGTATGC1300.29943567891281814TruSeq Adapter, Index 21 (97% over 35bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC860.19808821835771045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGG150.0029939427192.9944145
GTGTGCG150.0029939427192.9944145
TCAGGGT200.0070785335144.7458145
CGAGTAC100.0077604363140.533191
TCGGACC100.0077604363140.533191
TGCTCAC100.007978749139.236453
CAGTCCG100.007978749139.236458
GACTAGG100.007978749139.236454
GATCGGA405.848051E-9105.399891
TCGATAC204.5133274E-4104.427332
CCTGATC204.5133274E-4104.427338
TAGGTTA300.00225891669.6182257
AGCTCTA300.00225891669.6182259
GCAGCTC300.00225891669.6182257
GACTCTC350.00416109159.6727644
GCACTAC350.00416109159.6727645
AGCACTA350.00416109159.6727644
GAGCACA754.86667E-755.6945768
ATCGGAA807.126109E-752.6999441
AATCCTA400.00705822652.2136652
Produced by FastQC (version 0.11.5)