FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-larvae-day5-RNA-EPI-99-2_S16_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-2_S16_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214
Sequences flagged as poor quality0
Sequence length67-151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC796.507413509060955No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC201.6474464579901154No Hit
GAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAA70.5766062602965404RNA PCR Primer, Index 28 (96% over 29bp)
TGGAGCAACAGCTATTACCTGGCATCCATATGTGTAGGGTATCTTCTGTG50.41186161449752884No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.41186161449752884No Hit
TGAAAATAAAACATCCACTTCAGATATACTATAGTGGTCATGTGTCAACA40.3294892915980231No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.3294892915980231No Hit
ACGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.3294892915980231No Hit
GACAAGGTTCCCGCCAAAACAGTTTTCACTTGGAATGAATTTTTCTATTA40.3294892915980231No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3294892915980231No Hit
GCATATTTGACATTTTAACAAAATATAAATTTTACATTTAACAAATAATG30.2471169686985173No Hit
CCGCTGCCTTAACAACCTTTCCCATATAATAAATAAAAACCCAATTAAAG30.2471169686985173No Hit
ATCGGAAGAGCACACGTCTGAACTCCAAGTCACGTTTCGGAATCTCGTAT30.2471169686985173Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTGAGAAGGCAAACATTGGGGGTATCACGAAAGTTTGTATCCAAGTGTAG30.2471169686985173No Hit
CCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2471169686985173No Hit
GCCAACACAGGCATAGCAAGCACCAGCAAAAAAGCAGTGATAGCAACTGA30.2471169686985173No Hit
CTTTTGCATAAAATCAGTTTGCATTTCTGAATGTTTTTAGTTTTTACGTA30.2471169686985173No Hit
GTCTGCTATACAGAGGGCAATATTATAGTGTTGTCTGCTATACAGAGGGC30.2471169686985173No Hit
ATTGGTCCGATGTTGAGTTTTAAAATACCGCTTAAACACGAGATGAACCG30.2471169686985173No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2471169686985173No Hit
GGCAAAGGCACTGTTTTTAGAATTATTCACAAATAATCTAACAATCAATT20.16474464579901155No Hit
AGAAAAATGGGATTTTGAAAATATATCCTTTTTGAACAATGAGGATATAA20.16474464579901155No Hit
GTTCTTCAGCACTTGCACACTTGATCTTCTCAACTTTACCCAGGCTTGCT20.16474464579901155No Hit
GTCTTATTAAATAAAGAGAAACGGAAAGAAACACGGACACTAACAGATAC20.16474464579901155No Hit
TTTCTGTACATTTATTTTGCTGTTAACAAATAGAACTGGCATTTGATGCA20.16474464579901155No Hit
AAAAAAACCTCAAAGAATTATTTCAGAAAAGGTATACAATTAAATGTTTA20.16474464579901155No Hit
GGCTTCCTGTGTTTGCTGCACCTCCAGGTCGGCCTCCTGTAGGTGGTCTC20.16474464579901155No Hit
GGGTACTTAAATTAGCTTGTGGGTACTTTAGTTGTGTTGTGGGTACTTAA20.16474464579901155No Hit
GTTCATGTTTCTTACAAATTTATTTAATTATACAAAAGAAGAAATATTAA20.16474464579901155No Hit
TCCACATCTTGATCTTGGAAAGTGAAAGTTGGAAACTTGGTCATATCCTG20.16474464579901155No Hit
GTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGA20.16474464579901155RNA PCR Primer, Index 28 (96% over 29bp)
ACCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCC20.16474464579901155No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCC20.16474464579901155No Hit
GTTTATCGCTGCTACCGGAGTTAAACAGGAACGGCAGCAGAACCAAAAGA20.16474464579901155No Hit
TCGTTATCGTTGGTGCTGTTGTCGTGGTCGTTGGTGCTGTAATCGTTATC20.16474464579901155No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT20.16474464579901155TruSeq Adapter, Index 21 (97% over 40bp)
CTAAGATTTATAAGAAAAGGCCCATTTGGGCCTAAATAATATATTACGCA20.16474464579901155No Hit
CATTTCTATGTCATATGTTATGATATTGGATTTTTCTACAATTATTTACT20.16474464579901155No Hit
GTTACTTGCATGTTTAACATGCTGCATCAATGGTGCCGCTGCCATCACGT20.16474464579901155No Hit
GTTTTCTCATACAATAAATCAATAAGTTGTCAACTTTGGCCGAGTCTGAA20.16474464579901155No Hit
GTCACCTGGATTTTTGTCTGGATGAAACTTCAGAGATAATTTGCGATAAT20.16474464579901155No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC20.16474464579901155No Hit
AGATGAATGACTATCATCTGGAGTAAGGCATCACCAGCTGGTAACCATTTCCTCATCATTGTCTTTA20.16474464579901155No Hit
ATTACCACTTCAATCACACCTACAACTATCACTACCACTACCACTACCAT20.16474464579901155No Hit
TAGGTCTCGTCGGTGTTCTCAACAAGTTGGTGGACTGAGAGAGTGGCGTT20.16474464579901155No Hit
CTAGCTACTACAACTGAATGTAATACATTTGCACAACTTCCAAATATATA20.16474464579901155No Hit
CGCAATTCAATTGTTTTGTCAAAATTTCTTTCTGACATTTTCTTTAGCTG20.16474464579901155No Hit
CAAATATCTTCTACAGGATTTCTCTTGAGGCACAAACTTAAGCTACCTTT20.16474464579901155No Hit
AGAGTAGTTGCGGTAATTGTTACGTAGATGTTCGGATGTATATTCCCCAA20.16474464579901155No Hit
TTCATCCACTGTTCTATATCCATCACTCAATAAATCAGATCTTAAACTAA20.16474464579901155No Hit
AACCAACTTCTGGGGAAATCCAGGTCATCACTTTGACCTGTAATCTGCAG20.16474464579901155No Hit
TTTTTTAGTTGTTAGTTTCCGAATCTGCCGTTGTTGCTGCTACTGTTTCT20.16474464579901155No Hit
GCAATTCGAAGGTGACAGTTAGTTTTATAATTTAGATTCCGTTTCGATTT20.16474464579901155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC100.0055556493150.434781
GTATGCC159.7038705E-5144.166672
TGCCGTC251.9639629E-8144.166675
TATGCCG159.7038705E-5144.166673
CCGTCTT251.9639629E-8144.166677
ATGCCGT201.411945E-6144.166674
GTCTTCT251.9639629E-8144.166679
GCCGTCT251.9639629E-8144.166676
CGTCTTC251.9639629E-8144.166678
GAAAAAA254.7329324E-428.83333420-24
TGCTTGA254.7329324E-428.83333415-19
GCTTGAA254.7329324E-428.83333415-19
CTGCTTG254.7329324E-428.83333410-14
TTGAAAA254.7329324E-428.83333415-19
CTTGAAA254.7329324E-428.83333415-19
TCTTCTG254.7329324E-428.83333410-14
TCTGCTT300.001373966524.02777910-14
TGAAAAA300.001373966524.02777915-19
TTCTGCT350.003368368820.59523810-14
CTTCTGC350.003368368820.59523810-14
Produced by FastQC (version 0.11.5)