FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-larvae-day5-RNA-EPI-99-1_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-1_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61304431
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5286160.8622802485516912Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2446380.39905435220498175Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA946400.083.600191
GAGCGTC1088500.072.359539
AGAGCGT1118850.070.483778
AAGAGCG1212800.065.144197
ATCGGAA1286300.061.8169822
TCGGAAG1307000.060.8325353
CGGAAGA1319800.060.3081974
GAAGAGC1405700.056.4756746
GGAAGAG1846400.043.427445
AGCGTCG1152800.030.839
CGCCGTA984300.023.57556245-49
GGTCGCC1063100.021.93167940-44
TGGTCGC1079200.021.64430640-44
GTAGGGA1068700.021.48076415-19
GCGTCGT1072250.021.38758910-14
CGTCGTG1071450.021.3699410-14
CCGTATC1095050.021.29122545-49
TCTCGGT1094650.021.25710135-39
GCCGTAT1107150.021.11739345-49
CTCGGTG1132050.020.71518735-39
Produced by FastQC (version 0.11.5)