FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-larvae-day5-RNA-EPI-99-1_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-1_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61304431
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT5131220.8370063821324759TruSeq Adapter, Index 21 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG2311350.3770282118759083TruSeq Adapter, Index 21 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA952800.081.732741
TCGGAAG1138000.068.8188863
ATCGGAA1200150.065.346252
GAAGAGC1233000.063.6659936
CGGAAGA1257600.062.4252474
AGAGCAC1340200.059.5971838
GGAAGAG1359150.057.8184135
GAGCACA1433450.055.9087379
AAGAGCA1463850.053.67797
AGCACAC1538350.023.7419789
TATGCCG999500.023.58255645-49
CGTTTCG1018200.022.88572130-34
CGTATGC1032300.022.76876645-49
GCCGTCT1076700.021.72100650-54
GTTTCGG1072500.021.71281430-34
ATGCCGT1092450.021.4554645-49
CGGAATC1085350.021.28891635-39
AGTCACG1111500.021.10011725-29
TCGGAAT1110150.020.76962935-39
AATCTCG1118300.020.65991440-44
Produced by FastQC (version 0.11.5)