Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R2

Basic Statistics

Measure	Value
Filename	Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20155
Sequences flagged as poor quality	0
Sequence length	35-151
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	344	1.7067725130240636	Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	98	0.486231704291739	Illumina Single End PCR Primer 1 (100% over 50bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	91	0.45150086827090047	Illumina Single End PCR Primer 1 (98% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78	0.38700074423220043	No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	62	0.30761597618456954	No Hit
CTTCTTAAGCCACCCACCATGTTTCATTACAGAAAAAAATTGAATTATCG	32	0.15876953609526173	No Hit
CTTCTTAATAACTAAGAACGAACTTAATAATTAAAGTACGTGTAGGCTGC	31	0.15380798809228477	No Hit
CTGCGCCATCCGATCTGCCGAGTCTTCTCTGTTTAGCAATGGCTGGTGTT	31	0.15380798809228477	No Hit
TGAAGGTTGTGTCTATATTGGGTGTGGCCATAAAACCTCTCCACAGAAGA	31	0.15380798809228477	No Hit
TGTATTTCTGTACATTTCATGAAGTATGGCATCAAATCTCAGAAGACAGT	31	0.15380798809228477	No Hit
TGCCAATTCACAAAGCGCGAACACTTATGAACTTTTATATCACATAAATT	30	0.14884644008930786	No Hit
GCCATATATTCTTAATAAACAGATAAGTGTTTGCTTACAAGTGTTATTAT	29	0.14388489208633093	No Hit
CTTGGATCAAGATGCGAGCCAAGTGGAGAAAGAAGCGTATGCGCAGGTTG	28	0.13892334408335402	No Hit
CAGTTTAATGATATTGCACGGTGACAATTGATTGTTAAGCTCAAGCAGTG	27	0.13396179608037706	No Hit
CATCCCTGGTCTAGATTTCTTACAGGGTCATGTCCTTGGTTTCTTTGATT	26	0.12900024807740015	No Hit
CCGGGAGCACGGTGGTTCTATTCAGATTCATTTTGATGTATTTTTCCGCC	26	0.12900024807740015	No Hit
GGAAAGAAGAGTTTTTATATGTTTTAAAGTGTTAGGGGCGTGACTTTTAT	26	0.12900024807740015	No Hit
TGGAAGAGTATATAGGCGCATCTTTTTCTATTTCGGATGGGGTGTATGGC	26	0.12900024807740015	No Hit
GGACACCCGCCTTAACAAACATTTATGGTCACAAGGAATCAGAAATGTTC	25	0.12403870007442323	No Hit
GGTCAAGCTTGCTGTACTCAAGTACTACAAGGTTGACGAGAATGGCAAAA	25	0.12403870007442323	No Hit
TTCAAAACTACCGCTCCAACAGGAGTGATGTCTAACAAGTCATTTGTGGA	25	0.12403870007442323	No Hit
GCCAAATATCGAACTTGACCTATATTTTATGATAAGACACACAAAATATA	25	0.12403870007442323	No Hit
CAGTAACACATTGTATGAATTATTATGCTCCCGCAAAGGAGGGAAGCATA	25	0.12403870007442323	No Hit
TGGCTTCTATCTGCCAGTGTGGTTACTTTAATCGGTTGACTTATCACTCC	24	0.11907715207144628	No Hit
CTTTGATTTAGCATGATAAAACAAGAAAACAGAAAAAAGAAAGATGTAGA	24	0.11907715207144628	No Hit
CTTACTAATAGTGGAAGATCAAAAAAATTGAACCATTTGTCTCCCTCCCA	24	0.11907715207144628	No Hit
CAGATGTGAAAGAAAATTTTAAAGAGGCCGCCCCATCCACATCCTTTGGT	23	0.11411560406846936	No Hit
TTAAGAACATTGGAATCATGCAGCATGTGATATTAAAGAACCAACTTTAA	23	0.11411560406846936	No Hit
TTGTAAGCTAACAGACAAAATTATAAATACAACCTGGATCCTTTGGTAAC	23	0.11411560406846936	No Hit
TGAAAACCTACAAAATAAATTTAATTCAAATTCTAAAAACAAAACAATAA	23	0.11411560406846936	No Hit
AAACACGCTCGTTTATCGTTACCACCCCGTCCAACGTATACGTTACATAA	23	0.11411560406846936	No Hit
TCAACATTATGCACTGCGTCTTCAGATATTTTTTCAGCATATGTCAGCTG	23	0.11411560406846936	No Hit
AAAAATTTAACAAGATTTAGACTGAGTGCGCATAATTTAGAAATTGAAAC	22	0.10915405606549244	No Hit
CCAGACTCCTGAACCGACACTTCCAAAAATTCTGCGCCGAGTGATTCTGC	22	0.10915405606549244	No Hit
GGGCTTCTTTGACTATAAAGAATTTAGTTCTTTATCATAATTTGGCTTTA	22	0.10915405606549244	No Hit
GTACCATTCACCTTGGTAACAACCTAGAAATCTACTGCGGTAACCATGAC	22	0.10915405606549244	No Hit
GCCTTTCCTTCTCTATATAATTCCTTGATTTAGTGTTGTCTATATAGTAA	22	0.10915405606549244	No Hit
GATTGTCAAAAAATTGACAGATGCTGATGCGATGAAGTACACAATTGTTG	22	0.10915405606549244	No Hit
GTTTTTAGATTCCCTTATAACTTGCCTAGCTCAAAAACATAAGGTGAATG	21	0.1041925080625155	No Hit
GGAACAAGCAACAGCCCTTCTCATGGCAGGGAGACATCTTCCAGAGTCCC	21	0.1041925080625155	No Hit
AGTAAATATCACTTTGAAAATGGCAGATTTCTTTATGCTGTATGCAAGAT	21	0.1041925080625155	No Hit
CTGCATCTTTGCAGTGTATGATCCAGTCAAATGTCACATCTTCTTCTAGA	21	0.1041925080625155	No Hit
CTAACATTTATATATATAAAATGGGCAAAATAGTTAAATAATTCAGAATC	21	0.1041925080625155	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGCT	10	0.0062461277	150.8871	1
CTTCTTA	10	0.0062461277	150.8871	1
CGACTCT	10	0.0062461277	150.8871	1
ACCGAGT	10	0.0079397755	139.2804	6
CGCTCGG	10	0.0079397755	139.2804	4
GGCGCTC	10	0.0079397755	139.2804	2
GGACATC	10	0.0079397755	139.2804	2
AAATCAG	20	4.4722835E-4	104.4603	4
GAGCGTC	60	0.0	104.4603	9
GATCGGA	45	7.838025E-9	100.59139	1
CGGAAGA	60	1.0913936E-11	92.8536	4
TCGGAAG	60	1.0913936E-11	92.8536	3
ATCGGAA	60	1.0913936E-11	92.8536	2
AAGAGCG	80	1.8189894E-12	78.34522	7
CGAAAAC	30	0.0016274952	75.44355	1
GAAGAGC	85	1.8189894E-12	73.73668	6
AGAGCGT	85	1.8189894E-12	73.73668	8
GGAAGAG	105	1.4551915E-9	53.0592	5
CCGTATC	40	4.4992157E-7	27.897615	45-49
GCCGTAT	40	4.4992157E-7	27.897615	45-49

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content