Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R1

Basic Statistics

Measure	Value
Filename	Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20155
Sequences flagged as poor quality	0
Sequence length	35-151
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	221	1.0965021086579014	TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	121	0.6003473083602084	No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	83	0.4118084842470851	TruSeq Adapter, Index 6 (98% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.30265442818159266	No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	58	0.28776978417266186	TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGCAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	50	0.24807740014884647	TruSeq Adapter, Index 6 (98% over 50bp)
TATGTGTTATTACAAATAATAATAAATATTCTATTAAAAGCTATATAACT	31	0.15380798809228477	No Hit
TAATATAATACAACCATCAATTTCCATTTTCAAAAAGAAAAATGAAAACA	31	0.15380798809228477	No Hit
CGACACACAAGGAGTATCGTTTACTTTTGTGAAGACAAACATTTTGTTTC	30	0.14884644008930786	No Hit
CCTCCGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAAT	29	0.14388489208633093	No Hit
TGTAAGAAATAACTCAAACACACATATTGTATAGTAGCACTAAGAGAATT	29	0.14388489208633093	No Hit
TGAGGTTTCACTGCTTTCATCAGAATGAGACCCTGCGTTGCTTAGATGCC	27	0.13396179608037706	No Hit
CACCTTCATAGTAGCCATTTTGATCCACCGACCCTGTAACTGTGACAATA	26	0.12900024807740015	No Hit
CAAACACTTATCAGCAAACATTTGTATGCTATTATCTGTCAGCAAAAACT	26	0.12900024807740015	No Hit
CTCCGTGAAAAATCATCGAATGGGAACACAAAGACAATATGCACATCTGC	25	0.12403870007442323	No Hit
GGAAGTCTGAACTCTGGTGAATGTCATTAGTCGTTTTGTGCAATGAAAGT	25	0.12403870007442323	No Hit
GGAAGCGACGCCAATCTACATACTCATTGTCGGACGATGCTCCCGTCGCT	24	0.11907715207144628	No Hit
AAATTGTTCAAAATGTTACTTGCTTGAGCTACCTTTATCAACACCAATAT	23	0.11411560406846936	No Hit
AAACTATTCAAATCGTACAAGTCAATATATTATTATAACAGAGCTGTTCG	23	0.11411560406846936	No Hit
GGGAGAGTTCCACGAAATACAAAATCAGATTCAATATATTTGTCATTTAC	23	0.11411560406846936	No Hit
GCAAAACTTTGTTACGCTTCTGATTTACTTCAACTTGAATTAACATTCCA	23	0.11411560406846936	No Hit
GTCGTGTACGATTTCTTCTGCAACACTTTGAGTAAACAAGACTGTCCAGT	23	0.11411560406846936	No Hit
CAATTTTGTTGCCACAGCAACCACAATTTTTGTCCGATTGTAAAAGATGA	23	0.11411560406846936	No Hit
CCTAAGAATAAATTGTTTGTGCTTTATGAATTTTGACTAGAAAGGATTGC	23	0.11411560406846936	No Hit
TTTTTCTTTACCACAGTCTTTAATTCACTAAACAAATCACAGAACACTAA	22	0.10915405606549244	No Hit
GGACAACATAATAGAAAATGATACTCCGATTCAACCAACCTCCCATTACA	22	0.10915405606549244	No Hit
TTTTAATCCTAAAAATCAGATTTATTTGTTAAGACAATTTTAAAACACAA	22	0.10915405606549244	No Hit
GTTTTATTTACTATTTGATAGATATGGAAAGGCTATAACTAAACAATGTT	22	0.10915405606549244	No Hit
TCACATTCTACCACATTCACCTAATGTCTTTGATCAAGGCACCTTATAAG	21	0.1041925080625155	No Hit
CTCGGTTGACATCTCCTTGATATCCTTGGCGTAACCACCAGCTGCCGCGC	21	0.1041925080625155	No Hit
ACCTGGTCATACACTGCAGAGATACAGTCTAGAAGGAGATGTGACATTTA	21	0.1041925080625155	No Hit
ATCAACTCCATGTTTTCGTAACAATAACATGCTAGTAATCGGGCTAGAAT	21	0.1041925080625155	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTT	15	0.005416089	158.0168	145
ACAATAC	15	0.005416089	158.0168	145
CAGCGAA	10	0.007821716	139.98015	8
TACGATT	10	0.007821716	139.98015	7
AGAGCAC	40	4.1836756E-11	122.482635	8
CGGAAGA	45	1.05501385E-10	108.87345	4
AAGAGCA	45	1.05501385E-10	108.87345	7
TCGGAAG	45	1.3258614E-8	93.3201	3
GAAGAGC	55	5.14774E-10	89.07828	6
GGAAGAG	55	5.14774E-10	89.07828	5
ATCGGAA	50	2.7548595E-8	83.98808	2
GAGCACA	60	1.02591E-9	81.65509	9
GATCGGA	65	5.010091E-4	47.949	1
TGCCGTC	60	2.3876055E-8	23.36481	45-49
ATATCTC	60	2.4256224E-8	23.330025	35-39
TATCTCG	60	2.4256224E-8	23.330025	35-39
GCCGTCT	65	5.5750206E-8	21.567518	45-49
CGTATGC	65	5.6637873E-8	21.535408	40-44
GCACACG	65	2.9389328E-5	21.535408	8
GTCACGC	65	5.6637873E-8	21.535408	25-29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content