FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-2_S15_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-2_S15_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93893611
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1317570.1403258417657406Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1106070.11780034745921103Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC636000.029.71649
AGAGCGT739100.025.8049668
GATCGGA732900.025.772721
AGCGTCG682750.023.6721449
AAGAGCG878250.021.5826537
TCGGAAG957350.019.886093
CGGAAGA979300.019.46864
ATCGGAA992500.019.2096612
CGATCGG98650.016.38659145
GAAGAGC1238150.015.70544056
CGCCGTA472750.015.32262245-49
GTCGCCG517200.013.88564940-44
AGAGCGC157300.013.576659145
GGTCGCC532050.013.57086740-44
TGGTCGC573900.012.7764440-44
CTCGGTG587050.012.50744535-39
GCCGTAT587600.012.464251545-49
GCGTCGG192800.012.384486145
TCTCGGT596850.012.29049635-39
CCGTATC619100.012.01550645-49
Produced by FastQC (version 0.11.5)