FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-2_S15_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-2_S15_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93893611
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1221190.13006103258719062TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1047190.1115294202499039TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA660850.027.2893731
TCGGAAG695150.027.1995373
GAAGAGC835100.023.1397276
CGGAAGA832950.022.9065784
AGAGCAC948850.020.300118
ATCGGAA902500.020.2605112
GGAAGAG957250.019.9851635
CGCAAAT432800.019.5442661
GAGCACA1135750.017.4586759
AAGAGCA1208000.016.2828437
CGATCGG101600.015.158174145
GGATCGG207500.013.916299145
TATGCCG539300.013.50787845-49
CGTATGC549850.013.09020145-49
GCGGCGT250500.012.9553521
AGCACAC1331800.012.6927219
GCCGTCT617100.011.78768450-54
GCAAATC884650.011.6132932
ATGCCGT650900.011.26839845-49
GTATGCC679550.011.00910545-49
Produced by FastQC (version 0.11.5)