FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-7_S54_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-7_S54_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62396
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2910.4663760497467786Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1040.1666773511122508Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN770.1234053464965703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT100.008451958136.6269
TTGACGC100.008451958136.6267
AGAGCGT904.656613E-1060.7226688
GAGCGTC801.2674718E-859.773889
CTAACCA350.00449412758.5547
GATCGGA903.169771E-853.2177541
ATCGGAA903.230707E-853.0897252
CGGAAGA954.915637E-850.33594
TCGGAAG1101.5578371E-743.4719123
AAGAGCG1152.2088352E-741.581837
GAAGAGC1901.113261E-525.167956
CGCCGTA558.637093E-619.88564145-49
CGGTGGT802.1636553E-419.27491145
GGAAGAG2509.2579474E-519.127645
CGTATCA653.63823E-516.82631145-49
GCCGTAT653.63823E-516.82631145-49
TCTCGGT653.6835994E-516.80202535-39
GTAGGGA603.8163288E-415.92691815-19
CCGTATC706.860301E-515.62443245-49
GTCGCCG706.936009E-515.60438140-44
Produced by FastQC (version 0.11.5)