FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-7_S54_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-7_S54_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62396
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCAACAATCTCGTA2000.3205333675235592TruSeq Adapter, Index 15 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN790.12661068017180588No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCAACAATCTCGTAT750.1202000128213347TruSeq Adapter, Index 15 (97% over 38bp)
CTAAACTTTACTTTATTACTTTCTTAATAAAAATATATAAGCTCGACAGG650.10417334444515673No Hit
GCCGGATTCGTCATATTCCTGCTTGCTGATCCACATCTGTTGGAAGGTGG640.10257067760753895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATCG200.006532701148.7801145
GGACGTG100.008312021137.390811
CGTGCTA100.008412607136.83954
GACGTGC100.008412607136.83952
CCGACTC100.008412607136.83953
TCAGTCC100.008412607136.83953
ACCCTAC100.00843282136.729755
CGCTGCC300.002424936768.419754
GAGCACA757.566996E-963.8072139
TCGGAAG801.2518285E-859.8672753
GAAGAGC801.2598321E-859.8192676
AGAGCAC801.2598321E-859.8192678
ATCGGAA703.4311415E-758.6454932
TCTAAAC350.004480658658.598467
GATCGGA808.390598E-751.5215531
CAGTCCA400.00757534151.3148084
GCCTTAA400.00759943451.273668
CTAACTC400.00759943451.273668
GGAAGAG954.8863512E-850.374125
CGGAAGA1007.271956E-847.893824
Produced by FastQC (version 0.11.5)