FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-3_S22_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-3_S22_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60799145
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3412190.5612233527297135Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1719460.2828098980668231Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA717050.069.38921
GAGCGTC777050.064.192239
AGAGCGT835700.059.7363438
AAGAGCG925250.053.9325337
CGGAAGA958050.052.125334
ATCGGAA972550.051.345392
TCGGAAG1020850.048.960933
GAAGAGC1152800.043.460046
GGAAGAG1558500.032.437295
AGCGTCG808050.031.0728389
CGCCGTA696250.021.76026245-49
GTCGCCG726900.020.74411640-44
GGTCGCC741450.020.475940-44
TGGTCGC765250.019.89648840-44
GATCTCG762700.019.7934730-34
CTCGGTG769900.019.74169735-39
GCGTCGT776750.019.41251610-14
TCTCGGT788150.019.28630835-39
GCCGTAT800250.019.0884545-49
GTAGGGA801950.018.8799315-19
Produced by FastQC (version 0.11.5)