FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-3_S22_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-3_S22_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60799145
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT3206030.5273149811563962TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1494850.2458669443460101TruSeq Adapter, Index 15 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT741630.12198033376949627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA742200.064.9279251
TCGGAAG851500.056.7394563
ATCGGAA937100.051.5840342
GAAGAGC947600.051.3299186
CGGAAGA987150.049.311284
AGAGCAC1033000.048.5420388
GGAAGAG1031700.047.0599635
GAGCACA1143100.044.1840979
AAGAGCA1169550.041.67697
CGCAAAT329800.022.3779581
TATGCCG733450.020.89840745-49
CGTATGC735250.020.6528845-49
GCCGTCT752600.020.14510250-54
AGCACAC1272900.020.108989
ATGCCGT786800.019.3556245-49
GTATGCC807500.019.11639445-49
AATCTCG824550.018.23783340-44
TCTCGTA851650.017.8510740-44
CTCGTAT894200.017.10150340-44
GCGGCGT171850.016.8241621
Produced by FastQC (version 0.11.5)