FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-2_S14_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-2_S14_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062016
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG113520.5505291908501195Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT61530.29839729662621434Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53470.2593093361060244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44780.21716611316304046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22750.068.02281
GAGCGTC24700.062.5396929
CGATCGG2055.311449E-1060.018845145
AGAGCGT26950.057.3183868
AAGAGCG29300.052.7211727
ATCGGAA30000.051.7198682
CGGAAGA30100.051.3199544
TCGGAAG31850.048.500173
GAAGAGC36150.042.7311366
AGCGTCG25150.033.849639
GGAAGAG52500.030.3388335
CCGGCCT1802.1093462E-423.028551
GCGGGAT3104.037065E-822.2856941
CCGCGAT1550.00239471922.2856941
CGCCGTA22000.021.92315745-49
GGCGCAC1903.0411428E-421.8165191
TTCGGGG4353.6730856E-421.213558145
CGGGGTT5153.6379788E-1220.1220321
CTCGGTG24350.019.85579535-39
GTCGCCG24200.019.7545240-44
Produced by FastQC (version 0.11.5)