FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-2_S14_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-2_S14_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062016
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT105160.5099863434619324TruSeq Adapter, Index 15 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58700.28467286383810797No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG58190.2821995561625128TruSeq Adapter, Index 15 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49750.2412687389428598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA24750.059.4506151
TCGGAAG28150.054.229213
GAAGAGC31700.048.1258356
ATCGGAA31050.046.9497152
GGAAGAG33250.046.2979775
CGGAAGA33250.045.910234
AGAGCAC34700.043.96518
GAGCACA38700.040.6639069
AAGAGCA38100.040.402447
CGCAAAT12450.027.3163361
TATGCCG23300.020.77634245-49
CGTATGC23650.020.5851745-49
GCGTTGA7050.020.482284
GCCCACG3750.004254206520.181736145
GCGGCGT5850.020.1692031
GCCGTCT24100.019.8650750-54
AGCACAC43400.019.6343489
ATGCCGT25800.018.97634945-49
GTATGCC26450.018.61445-49
TCTCGTA26400.018.28016340-44
Produced by FastQC (version 0.11.5)