FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-116-7_S53_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-7_S53_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT80.4662004662004662Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG80.4662004662004662Illumina Single End PCR Primer 1 (100% over 50bp)
TAAAATTCTACCTGCCCTACATAAATACCATGTAATTGGCATGTCTATGC50.2913752913752914No Hit
GAAAACTTCCACCTTGATTATAAAAAAATATGACTCCATTTTTGAAAAGT50.2913752913752914No Hit
ATTAATGTAATAGATTGCTAGTATTCTATATGAAATCTGCCTTTTGCAGC40.2331002331002331No Hit
TGTTATATACATGTAAGATGTGTTGATTGTGCATAATTACCTGTAATGTG40.2331002331002331No Hit
TTTTGAGGGCTCTTTAATTCCTACTTTGTTATTAGTGTTAGGGTGGGGTT40.2331002331002331No Hit
GTTGACTTGGGATGTAGATGAACTTCAATGGTTTAGTTCCAAGATGCAAG40.2331002331002331No Hit
ACAAATGATTTCACATATGTACTGTAATTGAAATATGTGAAATCATTTGT40.2331002331002331No Hit
GTATTTTTCAGTTCTTCAGTTGGAAGAGTATATAGGCGCATCTTTTTCTA40.2331002331002331No Hit
TGAACACACTATTTTGCCACGTTACCTATAAATATATAACACCCGGTATA40.2331002331002331No Hit
TGACCTTTACATGTAAAGAAGATAAGTTTCTTTCAAGCAACAGTGATATG40.2331002331002331No Hit
GTGGGTTTTAATCGCTTTAAATGTCTATAGTTTGTCAGTGACTTTAAATG40.2331002331002331No Hit
GACCACCCCAACATACGGTGACTTGAACCATCTCGTCTCCGCCACAATGT30.17482517482517482No Hit
CACATCAATACTAGTCAGTTTATTAGACCATTATACGAGTTGGTGAGCCT30.17482517482517482No Hit
TTCAAGTGTAATACTGCCACTTGTATCCTTGATGGGTAATTTCAAAGGAA30.17482517482517482No Hit
AAAAGTTGGTATAGCATATACTGCAATAAGACATCACGGGAGTGACTTTT30.17482517482517482No Hit
GGGGTGAGTGTGACGTGAAGTCATTATGCGATTCGGTTTTTTAAAAAGCG30.17482517482517482No Hit
TTTGTCTCGGAACAGTTAAAGTAGGTCAGATTTCCCAAAGTTTTTTGCAG30.17482517482517482No Hit
TATTTACTAGTTTTTACTGTTAAATTTAACACGTCACTCATCATGTTCTC30.17482517482517482No Hit
TGTAGAACTAACCATAAAGACATTGGAGTCTTATATTTTATCTTTGCTAT30.17482517482517482No Hit
TGACTGTCTTTCTTCAACATTTGATGCATAATTTCTTTGATCAGTCATTG30.17482517482517482No Hit
GATTTTTCACTTGGATAGCAATTTAGCTATAGTTTAGCTTCCTTAAGCAG30.17482517482517482No Hit
GTTTATTCCAACAACTGCGTTATTTAAAAACACTTTAACAAATTAATTGA30.17482517482517482No Hit
CACGCCTTCACCGGTAGGCGGAGACAACGATTGCAGCATGCTTGTAAAAG30.17482517482517482No Hit
AACCAAACCATGTAACCAATGAAATGCAAACTGGTCCGTTTTAGCTCTGC30.17482517482517482No Hit
GCGAGGTGTACGTTTCAACATTCATGACGTAACCTTACACGCTGATGCTA30.17482517482517482No Hit
AAATTCTATGAACAATTTGGGAAGAACCTTAAGTTGGGTATTCATGAAGA30.17482517482517482No Hit
GTGAAGTCATTATGCGATTCGGTTTTTTAAAAAGCGCAGTGCTTTATGGG30.17482517482517482No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30.17482517482517482No Hit
GGATTCCTCATCTTCCACTCCTTCGGTGGTGGTACCGGATCCGGATTCAC30.17482517482517482No Hit
GGTGCAAGAATAAACATCATGTACAAGCGTTTCAAAGACAATTTCGATCC30.17482517482517482No Hit
GTGACTGTCTCATCTATAAATTAAGAAGTTTTCGTTTAAGTGAAAAGTCT30.17482517482517482No Hit
CTCTCATCTGCGAAGTCACTTCTGTCTTTCTACATTCCCATTGTATTTTT30.17482517482517482No Hit
CAGCCTTATGAAGCCTAACGGAATTTTGTTAAACATTAAAATGACAACATCTGAACTATTGTACTTGTGGAT20.11655011655011654No Hit
GTCCTAATGTTAAGGATGGCGAAAATGTGTTTGGTGTGGCCCACATCTTT20.11655011655011654No Hit
GGGAAAATCACCAATACTCAGAGAATTATTGCAGCACTGCCCACTGTGAA20.11655011655011654No Hit
CCACATTCAACCAGAGTGGTATTTCTTATTTGCTTACACGATCTTGCGGA20.11655011655011654No Hit
GTACGTCATGTATCGGACATTCGAGCAGACGACGCGATTCAAGATGGTCA20.11655011655011654No Hit
CCTGCATGTGCAAAATCAGCATTTTGAGCCAAACAGATGGGCACGAATAA20.11655011655011654No Hit
TGTATATATTCTGCCTATCAGTACTTTAAAACTCTGGTGTTATACTGACC20.11655011655011654No Hit
GTCGCGAGATGCAGAGTACGATTGAAGAAGAACAGCGTAGCACAAATGAA20.11655011655011654No Hit
TCTTAATGTAACATATGCATTGGTGTGTGCACAACTGCTTTGCAATTTCA20.11655011655011654No Hit
TTTAGGAGGAAGGAATGAAGTATATCGCCTGCTAGAGGTCGATAGTCGGT20.11655011655011654No Hit
TGTATACAGAGGTTGCTACTTCGGCTTCTACGATACCTTGAAACCGATAA20.11655011655011654No Hit
GTTAAATGTCATTTGGCTGACATTTTATGTAAATCTGGGTGGAGCTACAA20.11655011655011654No Hit
TTTGTGAAGACCCTCACAGGCAAGACCATCACCCTTGAGGTAGAGGCCTC20.11655011655011654No Hit
GTCCAGAATTTTAATGCTCTTTCTTTAGCTATAATTGACAGTGGATATCA20.11655011655011654No Hit
AAAAATGTTAGCAGTTCTGGGATAAAGATATATTCGATATTGAAAAACTG20.11655011655011654No Hit
CCTCAGACTTAGAGGTGGTGCCAAGAAACGTAAGAAGAAGAATTACACCA20.11655011655011654No Hit
GCGAAATTCCGCCTCAAGCCGGTCTCAACCATTTAAAAGTCGCTCAGGAG20.11655011655011654No Hit
CTCTATTTTTACTAGTTGGGTTAGTTCGGACTTACATGAATCACTTTGCG20.11655011655011654No Hit
GGCTCTCATCTGCGAAGTCACTTCTGTCTTTCTACATTCCCATTGTATTT20.11655011655011654No Hit
GTTACATTTGTATCATGGTTTACAGTTTTTATTGAATAGAGTGTGTTTGA20.11655011655011654No Hit
AGCGGTAGAATGGGTTGGACCTAAAGAGGGAGAAAGGAATATCAAGATAA20.11655011655011654No Hit
CTGAGTATTGTGCTCATACTTCGGACTGTTCACAGGAGCACTGCCTTGCG20.11655011655011654No Hit
CTTGCTCCATCCACCATGAAGATCAAGATCATTGCTCCACCAGAGAGAAA20.11655011655011654No Hit
ATTAGGGTCAGTTTTAAAATTGTATGGCGTTTTGGTGCTGAATAATAACT20.11655011655011654No Hit
TGAAGAAAGAACTGATAGAGATCCTGCAGAAGGTGGTTGGTGAACACAGG20.11655011655011654No Hit
AAAATTTTGTATTTAATGGAGATGTACAGAAGGGAGTATGGGATAAGGTT20.11655011655011654No Hit
GTTGGCGCTTTTCATTTTTGTTGTCTGTGGCTCAGCAATATTTCAAATCA20.11655011655011654No Hit
TTTTGCTAATTACAGAGTTATTGCCCTTTGATATATTTATTTGTCCGGAG20.11655011655011654No Hit
CTCAAGTGTAATACTGCCACTTGTATCCTTGATGGGTAATTTCAAAGGAA20.11655011655011654No Hit
GTCATGTTTATGCCATAGAGGGCTAACAGGCGCTCAGTTATACTAACCAC20.11655011655011654No Hit
GTGAACTCGAGGGTGAATACGACAACGAACAGCGCCGCCATGCTGAAACA20.11655011655011654No Hit
TGGATGTAAAAGCCCTTCTCTTAACAGTTGTGCTTCTGGCCTCCTACCAC20.11655011655011654No Hit
CAACAATAAAGGTTTTCCCGCCAAACATTTGGGATATAATGTCAAGACCC20.11655011655011654No Hit
TTTAAATTTCTCACGTTTTTCTACAGTGTAGTGTGTGATCGGCGAGCCTC20.11655011655011654No Hit
TTCATTTTTCAATCCCTTTTGGGATTTAAGGGTCTTTTGTAATTTTGTAT20.11655011655011654No Hit
TGTTGTTGTTTTTTATTTTTAATATAATAAACTGACGCTAGTTTATTATA20.11655011655011654No Hit
TGGCACTGGTGTTTTATGATCACTAACCTCTCGCCACTGAAGTCGTAGGT20.11655011655011654No Hit
CTTCACATGGGAAGATCTGGCAAGTGTTGACCAGTTCTGTTTAGCAATTC20.11655011655011654No Hit
CTGGTGAGGAAAGGGACATTGTTTGTAAACAGACTTCTGTCTAGCTTTCA20.11655011655011654No Hit
CCAAAAATATCCTGTTTGTTATTGGACGACCGGATGTCTACAAAAGTCCA20.11655011655011654No Hit
TGGGACGATATGGAGAAGATCTGGCATCACACCTTCTACAACGAGCTCCG20.11655011655011654No Hit
CACTTTACCAATCTCGTACATTTGATATGCTCGTATGTTGGAACTCCAAC20.11655011655011654No Hit
GGACAGCTATGTAGGAGATGAAGCCCAGAGCAAGAGAGGTATTCTTACCC20.11655011655011654No Hit
GGCCAGTATGCGGGTTATGACCAGGATGGGCACCAAAGTTCTCAATACAC20.11655011655011654No Hit
TGCACACTCCAGATCGTCAAGGGTGAAGGATTCATGTCCTTGATGAAGGG20.11655011655011654No Hit
TGACAGTTGACTTGGCTGGCATTTTGTAATGACCCATTTATCCCACCATT20.11655011655011654No Hit
GCCAAACCTTGGACACAATACAAAGTCTAAATCTCAGTGGGAACCAAAAG20.11655011655011654No Hit
GTGTATCTTCCTTCGAATGTCAAATCATTTTTGCAAAATAAATAAGTTAA20.11655011655011654No Hit
ATTTGAGTCTGAGAATCGAACTCCGTCTCAAATCTAACATTTTTGTAAAA20.11655011655011654No Hit
CGAACTTTGGAATTGACTTTGAAGCCACATTAGACGAAAAATGCTCGAGT20.11655011655011654No Hit
TTGCGTATCTTTGTACCAATTCAATTATAATATGAACAAGTTATGACCAA20.11655011655011654No Hit
TGCAAAGACAGCTTTCAAGAGCAAATGCTGAGGCCCAACAATGGCGTACT20.11655011655011654No Hit
ATTACAGATAGAGATCATGGACATCATACGGAAGGTCTGTAAGACTCTAC20.11655011655011654No Hit
TTCAAAACTACATTTGTGGGTTTGGAAGATTTGGGTTATGTGCATAAAAG20.11655011655011654No Hit
CTGCTTGTACCTCATCACGCTTGATTCATGTATGTCTGTAATTGCGTATA20.11655011655011654No Hit
CTTAACCAAAACATTGAGATCCCATATGAAATACTGAATGTAGCGATCGA20.11655011655011654No Hit
GAGTTGGTATCTAGATCAGCTGCCCTCATCCAGCAGTCTACAACAGTGAA20.11655011655011654No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11655011655011654No Hit
CGAGAACCGTGCTGCTGAGGCTGAACGTGTTGTCAATAAGTTACAAAAGG20.11655011655011654No Hit
TGACAACAGACGATGGTAGATCACAAACAACTTCTTTTCTTAAACCACTG20.11655011655011654No Hit
GTTATTGAAGCGTCAGATCTTGCAATATCAAAGTGCGACACTTGGTCTGT20.11655011655011654No Hit
AAAAGCTGGACCCAATGACGTTAAAGTTGAATTTGCTGGAAAAGAAGTTC20.11655011655011654No Hit
GTTCGAGTGTGGTTTTGACCCTTTGAGAAGGTCTCGATTAACTTTTTCAT20.11655011655011654No Hit
TCATGAGCAAGACGAACAGGCCACCATTGTCTCTTGCAAGATTGGTACGA20.11655011655011654No Hit
AAAAAAGAAAAAAATCTTCTTCATAGACAACAACCGGTCAGTTAAATTTG20.11655011655011654No Hit
TAACTGTAAAGCTCTTCAGAACGCCGTAGACCAGGCTTCGCAGAGAGAGG20.11655011655011654No Hit
GTTGATCGTAACTTGGAGGCAAATACTTCCAGGAAACATGTAAGAAGTGT20.11655011655011654No Hit
TCTGTCTGTAGGATTTTATTAATAAATAAATATGTGTGACGACGAAGTTG20.11655011655011654No Hit
CCTGGACATCGTGATTTCATCAAGAACATGATCACAGGAACCTCGCAGGC20.11655011655011654No Hit
CGGACAGACAGATTTTAGGCAAAATAACCAAAGATGGTGTGTTCCTGGAG20.11655011655011654No Hit
GAAGTTTCCACCTACATCAAAAAGATTGGTTACAATCCCAAAGCTGTTCC20.11655011655011654No Hit
CGCTTTTTATAAATCTTTGAGTTTCGCTGGTTGGGCTGGCTCCAGCGGAC20.11655011655011654No Hit
CAAACTTCCATGGCATGGACCTCACCCGTGACAAGCTCTGCTCCATGGTA20.11655011655011654No Hit
TAGTTGGGATCCTAGTAGGAGGTTTTCAATGGTTGAAATTAGTTGTAATA20.11655011655011654No Hit
TGAACTTGATGTCAACCATAAACAAATGTATCGTGTAAAAGAATAATATT20.11655011655011654No Hit
GGCAGCAAAGGGTACAATGATGTCATATCGTTACTGAATAGAACCTAAAA20.11655011655011654No Hit
TGACTAGTTGCACTTTCTTGATATTTGAAGGCTATCATAGACTGCATTTA20.11655011655011654No Hit
TCCACATTCAACCAGAGTGGTATTTCTTATTTGCTTACACGATCTTGCGG20.11655011655011654No Hit
GTTTGGTGGTGATGTGTATGGTTCCTGTCTCAATGTTGAGGCTGATATAT20.11655011655011654No Hit
GAAACAATCACCACACCCTCCCAGGTAGCTGCGCTCGTAGACTCCATAAC20.11655011655011654No Hit
GTTCAGCGGGTTTAAAATATACCCTGGCCATGGCAAAAAGGTGGTCAAAA20.11655011655011654No Hit
ATCGCCTGCTAGAGGTCGATAGTCGGTTTGTTTTGCCTAGAGGGGAGAAT20.11655011655011654No Hit
TTTTACCATTATTTGAGTGCGCGGTTCTTTTCCTCGTTTTCGGTATGATA20.11655011655011654No Hit
GGTCCATCCATTGTTCACAGGAAGTGCTTCTAAATAATAAACTGACAATA20.11655011655011654No Hit
CAAACATGACCTATTTGAACGAAGCCTCGGTGTTGCACAACTTGAGGTCC20.11655011655011654No Hit
CATGCAGCCTCTAGCTTGAGTGACTAACACTCTATCACCCTGCTTCAGTTATGGTTGCCCAATTCAAATGTTTAA20.11655011655011654No Hit
TTATTCTAGCTTTGTTGAAATGAGATGCTGAGAAAACCCTTTACGATTTC20.11655011655011654No Hit
GTATAGTTAACCACTGACTTCAGAAAATACACTTTTTGTTGTTCTTAATC20.11655011655011654No Hit
GCCAGAAACCCGTCCCTCAAGCAGCAGCTGTTTACATATGCTATTCTAGG20.11655011655011654No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)