FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-116-4_S29_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-4_S29_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90789803
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2496890.275018770555103Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2044500.2251904875264461Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1046270.11524091532614075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC872200.041.107958
AGCGTCG894150.040.1685689
GATCGGA775150.038.914841
AGAGCGT956750.037.5983737
AAGAGCG1093800.032.9453546
TCGGAAG1126700.032.584352
ATCGGAA1155050.031.2194231
CGGAAGA1197850.030.2942313
GAAGAGC1447650.025.2710155
GGAAGAG1911850.019.2705764
CGCCGTA728650.018.45117645-49
GTCGCCG797500.016.85252240-44
GGTCGCC809950.016.67567440-44
GCCGTAT826200.016.47916845-49
CCGTATC836050.016.4061545-49
TGGTCGC841550.016.11872740-44
GTAGGGA860150.015.62670415-19
GCGTCGT858950.015.61741810-14
CGTCGTG858150.015.53341910-14
GATCTCG875650.015.49149230-34
Produced by FastQC (version 0.11.5)