FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-116-4_S29_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-4_S29_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90789803
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2367520.2607693729658164TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1872230.20621588968532073TruSeq Adapter, Index 5 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1007180.11093536572603863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA766250.037.0186351
TCGGAAG979300.036.666162
CGGAAGA1083200.033.3976483
GAAGAGC1107750.032.707655
ATCGGAA1118650.031.9788461
AGAGCAC1171650.030.8822867
GGAAGAG1193050.030.305114
GAGCACA1332100.027.3552638
AAGAGCA1397350.026.0580336
AGCACAC1499700.024.3536749
GATCGGG178200.020.965704145
CGATCGG145400.018.887922145
TATGCCG751950.017.90425745-49
CGTATGC777400.017.49560440-44
GCCGTCT862150.015.79770745-49
CGCAAAT402800.015.5796881
TGCCGTC897600.015.46767645-49
ATGCCGT900250.015.12758345-49
TCGTATG940850.014.65829840-44
GATCTCG914950.014.65704635-39
Produced by FastQC (version 0.11.5)