FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-116-3_S21_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-3_S21_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630138
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT75031.1906915628005295Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG67031.063735245295475Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29210.46354925429033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11500.084.568581
AGCGTCG17350.058.226999
GAGCGTC17500.057.3378528
AGAGCGT18050.055.5907177
AAGAGCG18900.053.4517636
TCGGAAG19400.052.4092832
ATCGGAA19600.052.0653461
CGGAAGA19450.051.9237173
GAAGAGC22500.044.5961045
GGAAGAG23050.043.5250554
CGAACGT950.006241937628.8373241
CCGCAAT1400.001392214424.460231
CCGTATC16800.023.67643245-49
GGTCGCC17000.023.39153740-44
GTAGGGA16900.023.34327115-19
CGCCGTA16900.023.2936945-49
GCGTCGT16900.023.26657910-14
GTCGCCG17300.023.14388340-44
GCCGTAT17250.023.13802345-49
GATCTCG17250.023.11293630-34
Produced by FastQC (version 0.11.5)