FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-116-3_S21_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-3_S21_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630138
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC65811.0443744068759542TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC62850.997400569399084TruSeq Adapter, Index 5 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29370.4660883806404312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11950.077.11551
CGCGCGT300.002386300868.770911
GAAGAGC18350.052.501935
TCGGAAG18200.052.1962742
CGGAAGA18850.051.1174553
AGAGCAC20750.048.405147
GGAAGAG20200.047.7011954
ATCGGAA20400.046.8586121
GAGCACA22050.046.1710668
AAGAGCA21450.044.9142386
AGCACAC23950.042.504849
GCGGTTT1450.001667412623.7141081
CGTATGC17200.023.53482440-44
GATCTCG16800.023.27595535-39
TATGCCG17300.023.24921645-49
GCCGTCT17700.022.801145-49
ATGCCGT17850.022.60949545-49
TGCCGTC17850.022.37956845-49
TCGTATG18150.022.07693140-44
CTCGTAT18950.021.43359240-44
Produced by FastQC (version 0.11.5)