FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72390
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2450.3384445365381959No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.3121978173780909No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1300.17958281530598147Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN920.1270893769857715No Hit
CTTCTATCGTGTACGATCTTCCTGCGCCCGTGAATATTTCTGCTATGTGA860.11880093935626468No Hit
CGTGTCTTCTATCGTGTACGATCTTCCTGCGCCCGTGAATATTTCTGCTA760.10498687664041995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGC150.004126499173.5624145
GGTGCCC350.00401111860.2694938
TGGTGCC400.00680414952.7358067
GAGCGTC553.7207865E-451.1377539
AGAGCGT658.477525E-443.2704058
TTGAGAT750.001713528437.5010157
CGGAAGA850.003166387333.089134
AAGAGCG950.005457246729.6060647
TGGTCGC300.001776321823.45437640-44
TCGTGTA900.00646649823.4381357
CGGTGGT458.40765E-418.75570335-39
CGCCGTA400.00939382817.59078445-49
CTCGGTG400.00941634717.58347335-39
GATCTCG600.005751420314.062880530-34
AGTGTAG900.00646649810.93779725-29
AGATCGG1200.00197198510.494471130-134
GTTTTTT9553.6737017E-48.1102741
ATTAATG2000.00536820157.031440720-24
TTTTTTA18703.832067E-45.6401933
Produced by FastQC (version 0.11.5)