FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72390
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.45586406962287607No Hit
GCCAATTCCACCCTAGGCGTATAATGTATTACTAAAAAAAACCCCCTTAC2140.29562094211907725No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2090.2887139107611548No Hit
GAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAA1640.22655062853985355RNA PCR Primer, Index 36 (96% over 30bp)
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1180.1630059400469678No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1010.13952203343003178No Hit
CTCAAATCCAAGCTACGATGTCAGGCCCAAAATATGGAACAGCTCTAAGT800.11051250172675785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATTG400.00659723753.1491662
CCCGACG650.008704673549.524094145
ATGTTCC700.00117477840.4946026
TAAGTGT800.002263803735.4327779
GAAGAGC900.00403205231.4958046
GAGCACA1153.581541E-430.8111119
ATCTGCC1153.581541E-430.8111116
GATATCT1403.1688298E-530.370953
GGAAGAG950.00525284729.838135
CGATATC1204.5958132E-429.5273172
TATCTGC1505.0736417E-528.3462225
ATATCTG1805.5179207E-627.5588284
CGGAAGA1050.00856348926.9964054
AAGAGCA1050.00856348926.9964057
TCTGCCC1359.1507274E-426.2465027
ACGATAT1400.001122265825.3442551
TGCCCCC1400.001131395725.3091269
GCCAATT1550.002030067422.8915841
ATGCCGT2553.2299085E-622.2323324
TATGCCG2603.8225844E-621.8047873
Produced by FastQC (version 0.11.5)