FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-115-5_S36_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-5_S36_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78509378
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7070150.9005484669614884Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT6268170.7983976130851528Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1218600.084.5003741
GAGCGTC1709650.060.0372669
AGAGCGT1781900.057.6928148
TCGGAAG1919500.053.9401053
AAGAGCG1907000.053.922717
AGCGTCG1751150.052.70739
ATCGGAA1969100.052.6380772
CGGAAGA2015300.051.4105344
GAAGAGC2243800.046.139946
GGAAGAG2640700.039.413835
CGCCGTA1641800.024.03886845-49
GTCGCCG1700600.023.19220740-44
GTAGGGA1694200.023.03941715-19
GGTCGCC1719750.023.02949540-44
GCGTCGT1701450.023.02166710-14
GCCGTAT1730100.022.91985145-49
TGGTCGC1733900.022.86966140-44
CCGTATC1747800.022.70925545-49
CGTCGTG1725800.022.65651310-14
GATCTCG1748150.022.59751130-34
Produced by FastQC (version 0.11.5)