FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-115-5_S36_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-5_S36_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78509378
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT6729500.8571587460545159TruSeq Adapter, Index 14 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG5937090.7562268548351001TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1132650.089.102681
TCGGAAG1767200.057.919553
ATCGGAA1846600.054.968772
CGGAAGA1885800.054.6573874
GAAGAGC1935300.053.2615056
AGAGCAC1955450.053.0866438
GGAAGAG1969450.052.3061265
GAGCACA2101550.049.6446089
AAGAGCA2148700.048.0139277
AGCACAC2275650.040.949699
TATGCCG1634600.024.26269745-49
CGTATGC1653700.023.8687245-49
GCCGTCT1726850.022.84669550-54
AGTTCCG1717900.022.81597530-34
TATCTCG1712150.022.66466340-44
GTATGCC1771650.022.41804945-49
ATGCCGT1783500.022.1347245-49
TCCGTAT1782200.021.76073835-39
CGTATCT1806400.021.45301835-39
TCTCGTA1847150.021.21486940-44
Produced by FastQC (version 0.11.5)