FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-115-4_S28_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-4_S28_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382165
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG130480.944026219734981Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT118000.8537330926481281Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22300.086.306731
GAGCGTC32100.059.9143189
AGAGCGT33000.058.280298
AAGAGCG34900.055.1074377
TCGGAAG35000.054.9539643
AGCGTCG32250.054.2338649
ATCGGAA35700.054.075552
CGGAAGA36250.053.0590024
GAAGAGC41100.046.794396
GGAAGAG49350.039.6776285
CGCCGTA31350.023.70832845-49
CCTTCGC1500.001882267923.2276525
CGTCGTG31850.023.1925310-14
GCCGTAT32500.023.08395245-49
GTAGGGA31950.023.03236215-19
GCGTCGT32100.022.92506610-14
CTCGGTG32950.022.88571235-39
GTCGCCG32350.022.84001740-44
GATCTCG32550.022.73707830-34
TAGGGAA32950.022.67173615-19
Produced by FastQC (version 0.11.5)