FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-115-4_S28_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-4_S28_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382165
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT117600.850839082164575TruSeq Adapter, Index 14 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG112550.8143021998097189TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19800.088.682681
TCGGAAG33400.057.20843
CGGAAGA34500.056.1928944
GGAAGAG36250.053.6608625
GAAGAGC36150.053.6164326
AGAGCAC36650.052.8849688
ATCGGAA34250.051.3129652
GAGCACA38600.050.5745629
AAGAGCA40700.048.1363687
AGCACAC42400.041.4377449
CTCGGAA4750.030.8327922
GCTCGGA5400.026.0135861
TATGCCG30750.024.31730545-49
CGTATGC32050.023.11331645-49
GTATGCC32700.022.9951845-49
AGTTCCG31950.022.96002230-34
GCCGTCT32300.022.80930750-54
TATCTCG31800.022.72027240-44
CCCGAAT1550.002175143222.6569961
ATGCCGT33450.022.39618145-49
Produced by FastQC (version 0.11.5)