FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-juvenile-OA-exposure-RNA-EPI-115-2_S12_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-2_S12_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3135
Sequences flagged as poor quality0
Sequence length37-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC591.8819776714513556No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC441.4035087719298245No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.8612440191387559No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG80.2551834130781499TruSeq Adapter, Index 14 (97% over 43bp)
CCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA50.1594896331738437Illumina PCR Primer Index 1 (96% over 28bp)
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.1594896331738437No Hit
CAAACATAAAAAACCTACGGTTTTAGAGCTTAAAGTATGGAAACTTTAGT40.12759170653907495No Hit
AGTTGGGATCCGTGGCGGTAAGTGTTGTGACGGTAGATCCTGAAGCGGAC40.12759170653907495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC1500.077.0408558
GTATGCC1550.074.555672
TATGCCG1550.074.555673
CCGTCTT1550.074.555677
ATGCCGT1550.074.555674
GTCTTCT1550.074.555679
GCCGTCT1550.074.555676
TGCCGTC1600.072.225815
CGTATGC1600.072.225811
TGCTTGA1501.8189894E-1216.37118115-19
GCTTGAA1502.1827873E-1115.40817115-19
TCTTCTG1603.6379788E-1215.34798310-14
TCTGCTT1553.8198777E-1114.91113410-14
CTGCTTG1553.8198777E-1114.91113410-14
GAAAAAA1655.456968E-1214.88289420-24
TTCTGCT1606.184564E-1114.44516110-14
TTGAAAA1606.184564E-1114.44516115-19
CTTCTGC1606.184564E-1114.44516110-14
CTTGAAA1651.0186341E-1014.00742915-19
TGAAAAA1752.6011548E-1013.20700515-19
Produced by FastQC (version 0.11.5)