FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-8_S58_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-8_S58_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19573
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1620.827670770959996TruSeq Adapter, Index 13 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG530.27078117815357894TruSeq Adapter, Index 13 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1890359168241966No Hit
GGAAGCGATCTTCAGCACACATCTCCACTTGGCAAACTGGGCGCCATCTT340.17370868032493741No Hit
CTCCTCTGCCTTGGCACCTCTAACTGTGCAGTGAACAGCAATCTTTTCAT340.17370868032493741No Hit
GCCGCCTGCCCAGAGAAGCTGTATCCAATATATGATCCGGTGATCTTTAA320.16349052265876463No Hit
GGCGCTTCGTGGGACTCATCCTCTACTTTTGGCACATTATCTGAAGTGTT320.16349052265876463No Hit
GCCGTGTTCACAGTCATGAATTCCGAATTTTCTTCTCGGACAATCAAAAA320.16349052265876463No Hit
CACGCTTGCAAATTTTGCTCTTTAAAAAAACGGTCTCCAAGTGTGTTTTA320.16349052265876463No Hit
GCCAAAGATCCTAACCTAAAATGAAAAAAGGACCAAAAAAATGAGACAAA310.15838144382567823No Hit
GTTTTTGTATCCGTAGATTCTCCTCCATATCTCGTTTATGTTGTTCGCGA310.15838144382567823No Hit
CAGACCCATCTGCAATACTAAATTGAGAATGGCACCTTCTGAGCTGGTAT310.15838144382567823No Hit
GGGGGTACCCCCTCAAACACCTTCAATCGGGAAATGGCCTCCTTACCACG300.15327236499259184No Hit
TATAATGGAGAATTGAATTAAAAGAGTAGAATGTTTATGGCTATGTTTTT300.15327236499259184No Hit
GCTTTAAGGAGCTTGTTTATTAATTTGCATATTGCTACTCCGTCTCTTAA290.14816328615950544No Hit
GGACACTCCTACAGGGGACTATTTTCATTGTTGAACAAAATGCTAATTAG290.14816328615950544No Hit
GTCGTTCACAACCATAGCATCCTTAGAAGTTCGCCGGTATCTCCCCACAG290.14816328615950544No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290.14816328615950544No Hit
CAGCATTTGGCTAATTTCTCATTTTTCTTACAAATAACATTTTAATATTT290.14816328615950544No Hit
GATAATGACAGTTATATAATAACCATAAAGTTAAAATATTAAAATAACAG280.14305420732641905No Hit
ATACACTCTCTCAATTACCAGACCATATGAGAAGGGCGAGATATTCAACA280.14305420732641905No Hit
GATGCAAGACCTTTACTCTTCAAGATTCTCAAAATCTTGTTTCCAGTAAC280.14305420732641905No Hit
TTCTGGTTCAGGAACAGTTTCCGGTTCTGGTTCAGGTACAGGTTCCGGTT280.14305420732641905No Hit
GCAGTATCGTCCTTATCTTATTTCGGCAACTTTGTAATAAACGGCATATC280.14305420732641905No Hit
GGAACGCTTAGTTAGTCCACATGCCTGTTTCAAACTATTACATACCTTGA270.13794512849333265No Hit
GCACTAATGTCTTCATTCAATCACACATGGATCAATAAACCTTTATAAAA270.13794512849333265No Hit
AGAAGATACCCCCGCCAGATGAAGAGAAAAAATCCCATAATCAACAGAGC270.13794512849333265No Hit
GTACACATTTATTAGATCAACTATTGAACAATACAAACACCATATTTTTA270.13794512849333265No Hit
GTAATTTACAATTATGACAGATGGGCGTTGTTTGTGACGTCGTCAAATTA260.13283604966024626No Hit
CAAAAAATGAGGTATTAAAATTACGATCTGTTAATAATATTGTAATAGCC260.13283604966024626No Hit
CAAAACACCTACATTTAAATGTTACAGATTTCATTATGCTTTATCTTACA260.13283604966024626No Hit
ATATGCCTCAGCCTCCGCCACAGTTACATGTCGGTCTTTTTCCATATCTA260.13283604966024626No Hit
ATTTTTGCAAAATAAATAAGTTAACAAAGTTAACATGTAAATAATTAAAA250.12772697082715986No Hit
CTACCTTTCCGTCATCGTCAGAAGTAACTGGACCAAGATCTCCAACGTCA250.12772697082715986No Hit
ATTTTTTATGTTTTTATTCTGTTTATATTCATTTATAACTACAATCATTT250.12772697082715986No Hit
CTAAAGCCTATATTCATAACTGTGTTTCAAGTCTAAAGCCTATATTTATA250.12772697082715986No Hit
GAAACAACAACAACAACAACAACGTCTAATGAATTGTAGAATACTCAAGG250.12772697082715986No Hit
CAAATACAATTTTCTTGAACGGTGATAATTTTATATGCTATAAGATAATA250.12772697082715986No Hit
CAACACATTGCAGTTTATCAATGCCAAAAAGAAGGAAGATTCCAGGTTCA250.12772697082715986No Hit
CTAAACTTTACTTTATTACTTTCTTAATAAAAATATATAAGCTCGACAGG250.12772697082715986No Hit
TATATATTATATACCGTCTATCACTTCATTAGAGAATATTGTGCGAAAAA250.12772697082715986No Hit
CAAACCCATCTCTGCAAATAACTGATAACTTTCCCACTTTAATCAGTGGT250.12772697082715986No Hit
TTTGACTGTATACTTTTGGCGGTGGCATATAACACATACGTGTACCATTT250.12772697082715986No Hit
CATCAGCAGCCTGGTAATCATACACAGCTGTAGCACATTGCCCATGTTCT240.12261789199407348No Hit
TTTTTTTATTTGTATTTTACAAAGAATCACTCATCCTCAGCCTTTGTGGC240.12261789199407348No Hit
TCTGGGTCCATCAGCCATATAGGAAATAAAACAGATAAGAAAAAAATAAC240.12261789199407348No Hit
AGAAGCTTACTAACTTGTCTTGAAATTATCAATTCCAAATATTCGACAAA240.12261789199407348No Hit
GTCCAAACTTCAGCACAGCTCGACCAGTCAGTCTACACTCTGCCAATTCT240.12261789199407348No Hit
GGTGCTTTCACGATGTTCAACAAGGAGGGTCAGGCAAGTCACGATGAGTA240.12261789199407348No Hit
CCGCCCTGCGCTGTGATGTCAGCGTTCCCAGTCTATCCCACTGATCACAT240.12261789199407348No Hit
TTATTTTACATTGATTGTGATACAAGTTCTTGCAATGTACAATTCTTATT240.12261789199407348No Hit
AAAACAAACTTCACTTTTGACAATTGGATACTTTTGGAAATACATATATC230.11750881316098707No Hit
CAGGGCTGAAAGCCTTCCATACGATGGTCTCACAAATGTTGGTGGCAATA230.11750881316098707No Hit
TCAAGTAATCTGTAAGATCTCTGCCAGCCAAGTCAAGACGGAGGATGGCG230.11750881316098707No Hit
TGACCTTTGAGGTATAGGTGTGGGTCTTACTCGCGACACATCGTTATGTT230.11750881316098707No Hit
CAGAATATTTCTTTGCATTGAGACCACGTGATTCGATCTTACAGCTTTGC230.11750881316098707No Hit
GCATTATCTTCCACTAAATATATGGACAAATTTCCACATATAACACTATT230.11750881316098707No Hit
CTTGGTGTTGTTTCCTGCATGGAACATCGAACCAATACTCGGTGTTGTTT230.11750881316098707No Hit
CGAACATACCTCACCATGTGCTCCTTCACCATCAGTTTCCACCGTACAAA230.11750881316098707No Hit
CCACCTGACCAGCAAGATGACATACCCCAATAGAATGATCGAATAATGTA230.11750881316098707No Hit
CAAGAAATAACATTAAATATAAAACAGTGAAGTTAAGAAAGCAAATAAAT230.11750881316098707No Hit
CTAGTTGCTGCCCTTTACACTTCCTTCTCTTCGAACTACTTAATTATATT230.11750881316098707No Hit
AAAAACATTTTCTATTAAACATGTAAATCTATCACAATATTATATGCAAT230.11750881316098707No Hit
TTATGAATTATATTTTATTGAAAAGCTAAAACATAATCAAAATTACCTTT230.11750881316098707No Hit
GGAAACCAAAAGTGGAAAGGAAAGAGTCCTAATTTTAGAAACAACCCTCT220.11239973432790068No Hit
TAAAGTTCCTTTTGTACAGCATCGACTACAGCTAAACCGTAATTAGTCAC220.11239973432790068No Hit
TTTTAATGTAAGTTTTGATATTGACGTTTGCACTGCACATAACAATCAAG220.11239973432790068No Hit
TTTACACAGATGACAAACAATACATTATCTACCCAAGCCAACAATTCTGA220.11239973432790068No Hit
TAATCTTTTGATACACAATTTCAACCGCAGCTCAATTTATTTTTTAAATT220.11239973432790068No Hit
CGACACTCATCTGGACACTTTTATTTTATAAAACATAAATATCTATTTTA220.11239973432790068No Hit
TTATTATATCGGTGTGAAAACATTTACTGGCCACCACGTAAACGAAGTAC220.11239973432790068No Hit
CTCTTTCTCCAATCGCAATACATTTCAACAAGTGTTATCCAAACCCGAAA220.11239973432790068No Hit
CAGGATATTCTATAAGATGGACATTCATTATTATGATGACAGGGCATTCT220.11239973432790068No Hit
CAAGGTTTTCCCGGGGCATTTCATCATATTTGCCAATGAGTTTGATAAAT220.11239973432790068No Hit
GAAGTTTCTGGAGGGAGTTCTCAGCAGAGTCGGCACGCTCCTCTGCATCT220.11239973432790068No Hit
GTTTTCCCGCACCAATCACAGAAGGCCTTTGACAACAGTGCAGGAACAGG210.10729065549481427No Hit
TGGATAGTAAGAAACAATGGTTAATGAATGGATTGGATTGATAAACAGTA210.10729065549481427No Hit
GCGTATCAAAACATATAAAGCACTTTCACTGAAAATCACAAATATCCAAC210.10729065549481427No Hit
CTTCCTGCGCTTAGCCTTAACACTCCAGTTAAAGTGTCTGCCGGTTGCAC210.10729065549481427No Hit
AATTTCTAATTTCCTATCAATATAAGATCTAATTACAAAGTGAAACAATC210.10729065549481427No Hit
TTCAAGTTTCATTTCACATGAAACGATCAGCTAAACTGATTGAAACCAAT210.10729065549481427No Hit
TACACAACCAGGTAGTACAACAAACATAAGAATCAGATAAAATATGGCAA210.10729065549481427No Hit
AGGACCGATTCATATCCGCTGGGGCAACTGAAGGCACCAGTCATGGGGTT210.10729065549481427No Hit
TTCTAAATGAAATTTGACACACGGTAAAGCAGCACAACTGATAATGTTAT210.10729065549481427No Hit
GCAATTCTTTAGCTACTTAGCAGAAGCTATATGTGGGATATCGAAAAATT210.10729065549481427No Hit
GCCAGCTTCTGGCATCTTTTTAAGTCTCTTCTCCACATCGCTCGTCGTGC210.10729065549481427No Hit
ATCAAACAATTGTATACAAACAGTTCTGACTTCTATTTCTTATCTAGTGT210.10729065549481427No Hit
CCTGTTTTAAGAGCCAGTCAAGAGAAGGAGCAAAATTGAACTTTTAAGAC210.10729065549481427No Hit
CAAAGACCAAACTAGCGTGCTGACTTTTCCTTTGGATTCTGTTTAAAGGC210.10729065549481427No Hit
GAAGACACTTGTATATCTTAATCCAGTGTTAAGCACAACGCTGTTCTATC210.10729065549481427No Hit
GGACAGTGTTGGCGTACAGATCCTTACGGATGTCGACGTCGCACTTCATG210.10729065549481427No Hit
AAGGACTCTTTGGAGTGATTGTCTTGTCGGCCTCGAGAGGTTTGGAGGCG210.10729065549481427No Hit
TGATCATTAAATGGTGCTGTGACCGCGAAATACTGCCAGTCGTCTTTATA210.10729065549481427No Hit
GTGAAAGTCGTCAATCAGTATTTAAAAACTTTCTATTTTTAGAATTATTT200.10218157666172789No Hit
CCACATTCCTCTTCACGGTCGTAATTTTGTTCCATCTTCATTTCGACTTC200.10218157666172789No Hit
GGGAGATATACCCTTGACTATGTGATACAATTGGAATTGGGTTACCAGGA200.10218157666172789No Hit
CGACGCGTCAAGGATGTTCTCCTCTGGTTCCCAAGTGCAATGCTTTGAAG200.10218157666172789No Hit
TTTTCCACCCAGTCTGAGTGCAACTGTTTTTGGTCTAAATCCAGAGTACC200.10218157666172789No Hit
TGTGCTTCTGCTTCATCAAGACGTACGCCGAGTTCCTTCATCTGCGCCTC200.10218157666172789No Hit
TTTTAAAGTTCAATGAAATTTTAATTTATATAAAGGTCTGTTTTGGCAAT200.10218157666172789No Hit
CTTGGCATAGTCTTGGCATATATGTTGTGTCTCGTGTTTTATTTAGTAAT200.10218157666172789No Hit
CGACAATATTCATCAAATGCAATATCTCTGTATTGACACCCAGGTCCCAT200.10218157666172789No Hit
CACAATACAGCAGACAATAAGCTTTAAGCTACAAAATTATACAACACGCC200.10218157666172789No Hit
ACGAAGAGCTAATTATGAGTCAACTGAGGAGCCCCGCACTCAAAAAGATT200.10218157666172789No Hit
CCCATTTTTAAGAAAAATTTGGATGATAATCTAGCAATTAATTTATAATG200.10218157666172789No Hit
TTTTGCGTCTAGGATTAAATAATCCTCTCATGTTGAAGCATTAAAAATAT200.10218157666172789No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACATT107.223981E-4309.27426145
TGTCCTG150.0024322912206.18285145
CTGACTC100.008086035138.4225
GACCCAT100.008086035138.4223
CTCGGTT100.008086035138.4227
TCTCGGT100.008086035138.4226
TTTGCGT100.008086035138.4222
CGTCAAT254.717367E-8138.4229
TCGGTTT100.008086035138.4228
AAGTCGT202.6184352E-6138.4225
AAAGTCG257.9435085E-6110.7375954
GAAAGTC257.9435085E-6110.7375953
GCAACAT454.6231467E-4103.09143145
AGTCGTC353.5089397E-798.872856
AGAGCAC301.9648967E-592.2813348
TCGTCAA407.771105E-786.513758
TCTGACT250.001112165483.053194
GATCGGA250.001112165483.053191
AAGAGCA250.001112165483.053197
TAGACTC250.001112165483.053194
Produced by FastQC (version 0.11.5)