FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-7_S50_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-7_S50_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104552369
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4296670.41095864599682097Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1832480.17526910365847376No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1679170.16060563869193628Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC1186400.051.4248859
AGAGCGT1191050.051.287378
GATCGGA1241750.049.5058751
AAGAGCG1383450.044.273537
ATCGGAA1479100.041.6302382
TCGGAAG1789400.034.464683
CGGAAGA1951250.031.6234174
GAAGAGC2100950.029.6846836
AGCGTCG1075500.023.1890669
GGAAGAG3034150.020.7721145
GTCGCCG965650.018.09404640-44
GGGCGGG488600.017.925398145
TGGTCGC1052450.016.7476140-44
CGCCGTA1061850.016.56730545-49
CGTCGTG1042850.016.53223410-14
GCCGTAT1093550.016.1973945-49
CTCGGTG1107200.015.96104735-39
AGCGCGC49350.015.907175145
TCTCGGT1133550.015.58156135-39
GTAGGGA1135400.015.37625615-19
Produced by FastQC (version 0.11.5)