FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-7_S50_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-7_S50_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104552369
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3841810.3674531755468879TruSeq Adapter, Index 13 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1827370.17478035337487188No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1576690.15080385218244075TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1287900.047.5712933
GATCGGA1252350.045.7519071
ATCGGAA1320950.043.453052
AGAGCAC1471400.042.0040328
GAAGAGC1486750.041.837876
GGAAGAG1511200.040.9204755
CGGAAGA1534750.040.1302454
GAGCACA1618850.038.5255139
GATCGGG176550.037.004227145
AAGAGCA1748450.035.5404747
GCCGGAT455900.030.4907151
CCGGATT641500.023.7951851
CGATCGG188200.022.154285145
GGTGATT832800.021.771345145
CGCAAAT615300.021.3715021
CGGATTC705300.021.2552552
GGATTCG719900.020.1850323
TATGCCG911850.019.39272145-49
CCTACCT337000.019.14757
TACGCGT481950.019.1369469
Produced by FastQC (version 0.11.5)