FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-5_S34_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-5_S34_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99976
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.6331519564695527No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1150.11502760662559014Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT350.00404258960.1676941
CTGAGGC350.00404258960.1676941
CGGGGTG350.00404258960.1676941
GGTATAC350.00404258960.1676941
CGGGAAT350.00404258960.1676941
CGGCATT608.187819E-658.496371
AAGAGCG608.2611205E-658.408587
CGAGACC502.3478361E-456.1565131
CGGCAGT651.3155317E-553.9966471
AACGATG750.00672530952.871883145
CGAACAT400.00685743852.6467321
CCATAGG400.006898317552.5677226
CGACATT954.0005034E-851.723111
CGACCAT553.7589297E-451.0513761
CGGTACT553.7589297E-451.0513761
CGAGTTT658.564164E-443.197321
CGTCCAC658.564164E-443.197321
AGAGCGT658.6277846E-443.1324928
CGACGAC700.001233226540.1117971
CGACCAC700.001233226540.1117971
Produced by FastQC (version 0.11.5)