FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-5_S34_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-5_S34_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99976
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5070.5071217092102105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG700.002068149671.18115145
CGGATAT451.3694094E-462.6262781
CGAAAGC350.003984046660.3896261
CGTAACC400.00675827452.8409231
CGAAAGT553.6912103E-451.2396851
TGATACC800.002374133835.1039286
CGGAGAT1251.5251369E-533.818191
GGAGATC850.003164605733.1052322
CGAATCC950.00541413529.6650811
TTCACCT1204.866976E-429.2532717
GCCATCT1307.7725254E-427.0030213
TGCACTT1050.0089781526.7458487
CGTGACG600.005798251414.04719360-64
Produced by FastQC (version 0.11.5)