FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-4_S26_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-4_S26_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7793
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.603105350955986No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG260.33363274733735404Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT140.17964840241242142Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA151.3133638E-4141.259741
TCAGCTT100.007668931140.348398
TTCAGCT100.007668931140.348397
GAGCGTC151.3480062E-4140.348379
AAGAGCG151.3480062E-4140.348377
AGAGCGT151.3480062E-4140.348378
ATCGGAA151.3480062E-4140.348372
TCGGAAG250.001028649284.209033
CGGAAGA250.001028649284.209034
GAAGAGC300.002121208270.174196
GGAAGAG400.006630121352.6306425
CTCGGTG200.00704010728.06967735-39
Produced by FastQC (version 0.11.5)