FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-4_S26_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-4_S26_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7793
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.4619530347747979No Hit
GAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA170.21814448864365454Illumina PCR Primer Index 5 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT120.15398434492493263TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT110.14115231618118826TruSeq Adapter, Index 14 (97% over 44bp)
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC90.11548825869369947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATATT150.0026416457198.95891145
GACACCT100.0076353042140.554843
GAAGAGC204.212088E-4105.416136
ATCGGAA204.212088E-4105.416132
GATCGGA259.964519E-484.8805161
AAGAGCA250.001022660484.33297
CGGAAGA250.001022660484.33294
AGAGCAC250.001022660484.33298
TCGGAAG300.002108875770.277413
GAGCACA300.002108875770.277419
GGAAGAG400.00659168252.7080655
GGGGGGG256.076119E-428.11096820-24
Produced by FastQC (version 0.11.5)