FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-3_S18_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-3_S18_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14715
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG810.5504587155963303Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT440.29901461094121645Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC230.1563030920829086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG151.5086727E-4137.064647
CAGCATA100.008306836137.064649
GACCATG100.008306836137.064646
CATCAGG100.008306836137.064649
CGGAAGA204.7407998E-4102.798474
GATCGGA204.7407998E-4102.798471
GGAAGAG204.7407998E-4102.798475
TCGGAAG204.7407998E-4102.798473
GAGCGTC204.7407998E-4102.798479
ATCGGAA204.7407998E-4102.798472
AGAGCGT250.001150783782.238788
ACTAAAA350.001670813574.76252135-139
GAAGAGC300.00237258368.532326
GTTCTGT400.007412819751.3992355
Produced by FastQC (version 0.11.5)