FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-3_S18_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-3_S18_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14715
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGAATCTCGTATG620.4213387699626232TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGAATCTCGTATGC350.23785253143051308TruSeq Adapter, Index 13 (97% over 37bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC300.20387359836901123No Hit
CCGCCCTCAGCCAAAGATCCTAACCTAAAATGAAAAAAGGACCAAAAAAA150.10193679918450561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT150.0024345883205.7201145
GATCGGA300.00234316268.74831
ATCGGAA300.00234316268.74832
CGGAAGA350.004316189358.9271134
GAAGAGC350.004316189358.9271136
TCGGAAG350.004316189358.9271133
AGAGCAC350.004316189358.9271138
AAGAGCA400.0073211651.5612267
GGAAGAG400.0073211651.5612265
GAGCACA554.0500154E-449.9987649
TTTTTTT3000.006284320313.74966051
Produced by FastQC (version 0.11.5)