FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-heart-RNA-2_S10_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-2_S10_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC431.1357633386159536No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC220.5810882197569995No Hit
GGCGACTGTGTCTTCAGTGAATTAAGCCGTGAAACAGCCTAACTTGAAAC60.15847860538827258No Hit
CTCATATCTTTAATGTGACTGCCTGCAATGTCATATGGAAAAAATTATAC50.13206550449022716No Hit
AAAGGATAATAGTAACACTACAGAAGAAGCGTTCTTTTCGCTAATTTCAG50.13206550449022716No Hit
CTTTTTTGAATCTGAGGACTGCTCCTTTGTATGATGCAGGTGCATTGTTC50.13206550449022716No Hit
CTTGACTTGTGTAACATAGCACCGTGATGCTGTACTCTGTGTACTTCAAA50.13206550449022716No Hit
AGCCTAAAAAACATCAATAATGTACAAACATTGTATTAGGCCATGTGTAA40.10565240359218173No Hit
CTGAAAGCGTCTTTGTTTACTGAACCTTTATCCATGTTGACATCTACAGA40.10565240359218173No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT40.10565240359218173TruSeq Adapter, Index 13 (97% over 40bp)
TTTCAGAACACTGCCAAGGCAAAGTTTAAGATCTGCATTTCATTAAGTAT40.10565240359218173No Hit
TGTCTTTGTACTAGAATATGTCATAATATCTCCATTATCTATTATTTTGA40.10565240359218173No Hit
GCAGAACAGCAATGTTTATTTTAATACTAAACTAATATCATGAAATAACA40.10565240359218173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC253.475543E-8141.662165
CCGTCTT253.475543E-8141.662167
ATGCCGT253.475543E-8141.662164
GTCTTCT253.475543E-8141.662169
CGTATGC253.475543E-8141.662161
GCCGTCT253.475543E-8141.662166
GTATGCC301.0320218E-7118.051812
TATGCCG301.0320218E-7118.051813
CGTCTTC301.0320218E-7118.051818
TTCTGCT255.6881265E-428.3324310-14
TCTGCTT255.6881265E-428.3324310-14
CTTCTGC255.6881265E-428.3324310-14
TGAAAAA255.6881265E-428.3324315-19
TGCTTGA300.00164880723.61036115-19
CTGCTTG300.00164880723.61036110-14
CTTGAAA300.00164880723.61036115-19
GAAAAAA350.004036185320.23745220-24
GCTTGAA350.004036185320.23745215-19
TCTTCTG350.004036185320.23745210-14
TTGAAAA400.00873072717.7077715-19
Produced by FastQC (version 0.11.5)