FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-gonad-RNA-8_S57_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-8_S57_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55661709
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG24931934.479188736371713Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT8335601.4975465449686425Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1725450.30998868539950863No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN713860.12824974526024704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3162000.0117.5998151
GAGCGTC3832100.097.025029
AGAGCGT3886300.095.7110758
ATCGGAA3951300.094.651642
TCGGAAG3960800.094.374153
CGGAAGA3989100.093.613034
AAGAGCG3987850.093.256947
GAAGAGC4188000.088.991936
GGAAGAG4356050.085.862665
AGCGTCG3824750.031.8846659
CGCCGTA3777500.026.41361245-49
GCCGTAT3795500.026.32051745-49
GTCGCCG3804050.026.29005440-44
CCGTATC3823050.026.19180745-49
TGGTCGC3827150.026.1433340-44
GGTCGCC3848150.026.06804740-44
GTAGGGA3790500.026.04343615-19
CTCGGTG3848600.026.01248235-39
TCGGTGG3858150.026.00054635-39
GCGTCGT3799400.025.94595710-14
Produced by FastQC (version 0.11.5)