FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-gonad-RNA-8_S57_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-8_S57_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55661709
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC24110954.331694163396959TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC7839891.4084889129077944TruSeq Adapter, Index 6 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1563010.28080524800271583No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT1555270.2794147049994459TruSeq Adapter, Index 6 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN693220.12454163058486041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3049200.0119.4505841
TCGGAAG3838950.095.176273
ATCGGAA3874650.094.51382
CGGAAGA4102800.089.2766954
GAAGAGC4244400.086.201956
AGAGCAC4367700.085.7140668
GGAAGAG4333000.084.459635
GAGCACA4479450.083.6236659
AAGAGCA4454250.082.173437
GATCGGC141800.046.910511
GGATCGG120000.029.659943145
AGCACAC4609100.027.1415589
TCGGCAG264000.027.0004673
CGTATGC4161850.025.75616340-44
TATCTCG4162850.025.41459335-39
GTCACGC4187000.025.39170325-29
GCCGTCT4158600.025.36029845-49
CGCCAAT4222350.025.04326230-34
TCGTATG4271450.024.9696840-44
TGCCGTC4234750.024.90426445-49
Produced by FastQC (version 0.11.5)