Geoduck-gonad-RNA-7_S49_L007_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	Geoduck-gonad-RNA-7_S49_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2305
Sequences flagged as poor quality	0
Sequence length	35-151
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	609	26.420824295010846	Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	204	8.850325379609544	Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	10	0.43383947939262474	No Hit
GTGAAAGCCAAGATCCAAGACAAAGAAGGAATTCCCCCAGATCAGCAGAG	8	0.3470715835140998	No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA	5	0.21691973969631237	Illumina Single End PCR Primer 1 (100% over 50bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	4	0.1735357917570499	Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGACTGTAGATCTCGGTGGTCGCCG	4	0.1735357917570499	Illumina Single End PCR Primer 1 (98% over 50bp)
ACGGATTCCTCTAGTACCCGGATGGTCGATGGGAACTGTAACATCTACAA	4	0.1735357917570499	No Hit
CAGACGCCGGTGTCACTCCTCTTGTTGATCTGCTGCTCAAGGCCGGTGAG	4	0.1735357917570499	No Hit
GTAGTGGTAACAGTCTCGGTGAGTGAAAACGTAATTACTCCCTTTGAGTG	3	0.1301518438177874	No Hit
CTTACATATGTACATAGCGTGATATTTTTTTTTAAATTACGGGGAATCCT	3	0.1301518438177874	No Hit
GTCAGGTATAAAACAACTTGATGATAAGATCGTTGACCTTAATGCCAAGA	3	0.1301518438177874	No Hit
CTTTGCTATTTGAGCTGGTATAGTTGGTACAGCTTTTAGAATATTAATTC	3	0.1301518438177874	No Hit
TCAATGTGCACCAAGTATCCGTTCCCGGATGGGTTCTACTACCTCGCTGG	3	0.1301518438177874	No Hit
CAAGGTTGATAGAAAGAGCTTTTTTGGGTTAATAGGATGTAAATTCAGGT	3	0.1301518438177874	No Hit
CTCCCACAAAAAATTATTCGCTCCTGACATGCATTTCCTGTGTTTTTTTC	3	0.1301518438177874	No Hit
TTTGAGAAGGAGGCACAGGAGATGGGAAAAGGATCCTTCAAATACGCTTG	3	0.1301518438177874	No Hit
GGAGAATTGATGATGTACATTTCAAAGATGGTGCCGACATCAGACAAGGG	3	0.1301518438177874	No Hit
TCAAGATGGTCACGGGCTTCTAACACAACAAGCGAAAACAAATATTTACG	3	0.1301518438177874	No Hit
AAATTACCTCAGGTCGAGAATTAAATAAAGGGGCACTATAGATAACAAAC	3	0.1301518438177874	No Hit
GTTTTTTGTACTACATTATTTAATTCCTTTTGTTGTGAGGGGTTTAGCGG	3	0.1301518438177874	No Hit
AGCATGTGTTAGAATTGTGTCTGTCAAATAAAAGTGTGAGAATGAAGACA	3	0.1301518438177874	No Hit
TGGTACTGGGTGAACAGTCTATCCTCCCCTTTCTTCAAATGTTGCCCATA	3	0.1301518438177874	No Hit
CTTTAAAACTGTTTGCTAACAAATTGAAAATAATCTTTTATTAAAAAAAC	3	0.1301518438177874	No Hit
AGCAAATATAATTGTAGAAAAGTTAGTGAATGATAATGGAAGTTCAGGTT	3	0.1301518438177874	No Hit
GATCGGAAGAGAGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	3	0.1301518438177874	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	70	0.0	138.10869	1
GAGCGTC	90	0.0	107.41788	9
AAGAGCG	90	0.0	107.41788	7
GAAGAGC	90	0.0	107.41788	6
TCGGAAG	90	0.0	107.41788	3
AGAGCGT	90	0.0	107.41788	8
ATCGGAA	90	0.0	107.41788	2
GGAAGAG	90	0.0	107.41788	5
CGGAAGA	95	0.0	101.7643	4
AGCGTCG	90	7.2759576E-12	30.69082	9
GTAGATC	90	0.0	27.62174	30-34
TGGTCGC	90	0.0	27.62174	40-44
GAGTGTA	90	0.0	27.62174	25-29
GTCGTGT	90	0.0	27.62174	10-14
GTCGCCG	90	0.0	27.62174	40-44
GTGTAGG	90	0.0	27.62174	15-19
GTGTAGA	90	0.0	27.62174	25-29
TCGGTGG	90	0.0	27.62174	35-39
TCGTGTA	90	0.0	27.62174	10-14
CCGTATC	90	0.0	27.62174	45-49

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content