FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-gonad-RNA-7_S49_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-7_S49_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC55924.251626898047725TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1988.59002169197397TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT482.0824295010845986TruSeq Adapter, Index 6 (100% over 50bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC331.4316702819956615TruSeq Adapter, Index 6 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC120.5206073752711496TruSeq Adapter, Index 6 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110.4772234273318872No Hit
ACAGTGTTCGCCCATCTTCCAACTGCTTGCCAGCGAAGATCAATCTCTGC80.3470715835140998No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG50.21691973969631237TruSeq Adapter, Index 6 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT40.1735357917570499TruSeq Adapter, Index 6 (100% over 50bp)
GCGGTTTCGAACTTCCATAAAGCGATATCAATGGTGATACCACGTTCTCT40.1735357917570499No Hit
AAATAGCATTTCTAAATATCCTTGATATACACGATCTATAGTAACTGTGT40.1735357917570499No Hit
GTCATCCCAAGAAAAGCTAAGCCGGTACTTTGACGAACGCCGTTTTTCAC40.1735357917570499No Hit
GGTTTGCAATGAGATTTCTTTATTGTTTAAGACAACACAATCATTTATTC40.1735357917570499No Hit
TTTACACTTCCTTCTCTTCGAACTACTTAATTATATTTCCTCGCCCAAAA30.1301518438177874No Hit
CGAAACCAGTCATACCGGACTGACTTGCGTAAATATTTGTTTTCGCTTGT30.1301518438177874No Hit
CTCTGTCGTGTATTAATGTATTTAATACACATGTTCGGTTGCGCCACTCA30.1301518438177874No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAATCTCGTATGCC30.1301518438177874TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC30.1301518438177874TruSeq Adapter, Index 6 (98% over 50bp)
TCAGAAACACTTCTTTCATGCTGATTGATATTGCGTTTCATTTGATTTTC30.1301518438177874No Hit
GTTCTCGTTGCCTTCTCTCAGTGTGGAATGCCCACTGTCTATATCTTCTA30.1301518438177874No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCC30.1301518438177874TruSeq Adapter, Index 6 (98% over 50bp)
CTTTGGAATTTCCGTCACGACGCACTCGGCTGTCGTCAACAACGAGGCGA30.1301518438177874No Hit
CGAGGGCGGAGCCTTAGAACTGTAGGAAGGCGGAGCTATGTAATTGTATG30.1301518438177874No Hit
GTTGGGACCCATAATTCTGACCTTCTGTCCTGTACCTACAGTACCAGAAA30.1301518438177874No Hit
GTAACTGCTTACATCAACTGCTATTTAATGATACTTGGCAAAATCAAACA30.1301518438177874No Hit
ATTCTGTGCATAAATTTAGGCATGTGTAGCACAACTCTCTTTACGGATCT30.1301518438177874No Hit
CACACCAATCAAATCTTTAATAGTAAAATAATTATGAAAAGGGACTTTAT30.1301518438177874No Hit
GCAAATACTTGTCTTACAGTGTGTTACTAAGAGTTGATATGTTCTGTTAT30.1301518438177874No Hit
GTTAGTTTAAAAGCAAGAACCCAAATCCCGCTTTATACATGTCATTTAAG30.1301518438177874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA550.0138.32611
GCTCGGA100.0076453138.326081
GAAGAGC900.0107.586966
GGAAGAG900.0107.586965
AAGAGCA950.0101.9244847
GAGCACA950.0101.9244849
CGGAAGA950.0101.9244844
AGAGCAC950.0101.9244848
ATCGGAA750.0101.439132
TCGGAAG900.099.9021763
GTCACGC950.027.66521825-29
ACGCCAA950.027.66521830-34
CAGTCAC950.027.66521825-29
CTCCAGT950.027.66521820-24
CGCCAAT950.027.66521830-34
AACTCCA950.027.66521820-24
GCCAATA950.027.66521830-34
AGTCACG950.027.66521825-29
AGCACAC1003.274181E-1127.6652169
ACACGTC1000.026.28195610-14
Produced by FastQC (version 0.11.5)