FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-gonad-RNA-5_S33_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-5_S33_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073
Sequences flagged as poor quality0
Sequence length49-151
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG161.4911463187325256Illumina Single End PCR Primer 1 (100% over 50bp)
GGTAACGTGGACTACTCCTTCCGGTGGTAGTGGTGCCTTGGCAACGTCAG90.8387698042870456No Hit
TTTATCCTTCTATATCAGTCCTTGATTTCATGCATAATTATGAGTAAATT70.65237651444548No Hit
ACCACACCCACATTCAGGAGCCCTGAGGGAAACCTCACTTTTGGCACAAC60.5591798695246971No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.5591798695246971No Hit
AATTCATGTGGATGACTATGAAATATTATGCATATTTGTTACTTTATGTT60.5591798695246971No Hit
TGATATTTGCTGCCATATTAGTAGTTGTTTATATAATTTTAAAGGGTTGG50.4659832246039142No Hit
CCTTATATAACATAGGCTTCTATTTCACCAACATTTTTCGAAGTTTTACT40.3727865796831314No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT40.3727865796831314Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCAATCATTCCATCATGTGACCAGTTTTGTACATCATCATTCCGTGT40.3727865796831314No Hit
CACGGAACCACAATAGAACCAGAAAACAAGAAAACAAGTGCGGAAGAAAA30.27958993476234856No Hit
CTGTAGCCCCAGCATCTGCAACTGTGACTCTTACGTTATCAGTTACAGCC20.1863932898415657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTTG100.0032358952177.23529145
CACAATA100.006316443143.476189
CGGAACC100.006316443143.476183
GAACCAC100.006316443143.476185
GGAACCA100.006316443143.476184
ACGGAAC100.006316443143.476182
CACGGAA100.006316443143.476181
Produced by FastQC (version 0.11.5)