FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-gonad-RNA-2_S9_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-2_S9_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1667.717340771734077No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC622.882380288238029No Hit
GAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAA291.3482101348210134RNA PCR Primer, Index 28 (96% over 29bp)
CCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC120.5578800557880056No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC120.5578800557880056No Hit
ACGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC90.41841004184100417No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC90.41841004184100417No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC80.3719200371920037TruSeq Adapter, Index 6 (100% over 50bp)
ACGCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.18596001859600186No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.18596001859600186No Hit
ATAACCAATCACTTCCATATCAAAATGTTGAAATGCGTCCAAAACTGTTC30.1394700139470014No Hit
GACAAAGTCATTTCTAAGCTTCTGTTGTTATAACATTGACAGTACTTGAC30.1394700139470014No Hit
AGTTGTCAAAGATAAGACGTCCCTCTTCAACACCTGTTACAATGGAGGCA30.1394700139470014No Hit
CGCCAACCCTCCTAGCCTGCACAGATAAAATCAACATTATCACCCCAAGT30.1394700139470014No Hit
TTTTTTTCAATGTTATCAACTTTCCATTTATTCCGGTATAGAATATCTAT30.1394700139470014No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30.1394700139470014No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC30.1394700139470014TruSeq Adapter, Index 6 (100% over 50bp)
TGTTGTGCTGGTTTGGCTTCCTTCTTTTTTGCCCCTTCCTTCTTATCACC30.1394700139470014No Hit
AACCGCTCCAGCACATAAAAACATCAATGCCTTAAACAAAGCGTGGGTAG30.1394700139470014No Hit
TAACAGACGATCAGTTAACCTAACCACTGTTTCCTGGGATTAACCAATAG30.1394700139470014No Hit
AAACAGTATAAACTTATGTATGAACTGGTTAAAGAATACCCCTGTAACAA30.1394700139470014No Hit
TTTTTTAAAGATTTAATTTTATTTAAAACATTGATTGAAATTCAGACGTT30.1394700139470014No Hit
CTTTGAAAACAGTTCAGGAAGAGTTCAGATTTCTCTGTCCGTCCGTCACA30.1394700139470014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCT100.006744508144.023253
ACCCCCC1101.7934135E-432.7325551
Produced by FastQC (version 0.11.5)