Geoduck-gonad-RNA-1_S1_L001_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	Geoduck-gonad-RNA-1_S1_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137
Sequences flagged as poor quality	0
Sequence length	35-151
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	110	9.674582233948989	Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	50	4.397537379067722	Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	15	1.3192612137203166	No Hit
GCCGTTCCTGGGCGGTTGAATATGCTGACTTTAAAGAGAAATCATGTTGG	11	0.9674582233948988	No Hit
CCCTCCCGCACATGCAATGTAAATAATGTAGGTTTTCATTCATTTAGTAT	10	0.8795074758135445	No Hit
TTTGCATTTGATTTCTCATGGCTGCACACTTGATTTCTCATGGATGCACA	9	0.79155672823219	No Hit
TATATCAGAAGAAAAGGCACAGCCAGTTGCAAGTTCTAAAAGGGTATCTC	7	0.6156552330694811	No Hit
GTTTCATGATCCAAACAAAGCTTCCTATATACATACATGTCACCTGACCA	7	0.6156552330694811	No Hit
TTTACGAATGGCTGTTTTCAAGACTGGTGGTAATAAAAAAAAACACAAAC	7	0.6156552330694811	No Hit
CTTTTTAATGTGTTCTTGGATTCTGATTGCTAGAATTTTGTTAAGGATTT	6	0.5277044854881267	No Hit
GAAATGGTGTTTATCTGTCTGCAACTAGTCTATCACAGAGCCTGATACCT	6	0.5277044854881267	No Hit
CACAGGTGAGAAATATATCTCAAGACTTAAACTTTCTGAATTCTATTCTT	6	0.5277044854881267	No Hit
CATGCTTCCATCCAAATCAACTTTGTTGATGTTGATGATACCACTGGTAG	6	0.5277044854881267	No Hit
TTTTAAGCTAAAACAGAACGGTGTTTGAAATTGGCGGAAAGTTATTTAAC	6	0.5277044854881267	No Hit
TTTTAATTTATATATACATGAGGGCTAATCATTATAGACCTGCATTGATA	4	0.3518029903254178	No Hit
GCGAGGAATATTAAACAATTTTCCGAAATAAAAAATAACTTTAACGAAAA	4	0.3518029903254178	No Hit
ATTTTAACTCATTGGTAATGGACATCATGCAAAATTGTCAATGTAAAGGA	4	0.3518029903254178	No Hit
GTCGCCGTGGGAAGGGATTGAGTTGAGGCGGGCTGGAATTGACTCATTCA	3	0.2638522427440633	No Hit
TTTTCAGTTAACCCCACAAGGTCATCCACAAAAACCGTTTAATCTAAACT	3	0.2638522427440633	No Hit
CTTGGAACCAACTGTTGTCGATGAGGTTCGCACTGGCTTGTATAGGCAAT	3	0.2638522427440633	No Hit
GGATTGTTAGGGGGTTTTGGGAGAATTGTTTGTACTTCTAGAGTAGGACT	3	0.2638522427440633	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.2638522427440633	No Hit
TTTCAATGTTCCAGCCAGTTGCATGACGGGTCCTATTTTACCTAGTTTAC	3	0.2638522427440633	No Hit
GTTGAGTTTTCCATTTGTCGTGCACAAGATGTGACGGATATGGAAACCCA	3	0.2638522427440633	No Hit
TAATACTTATAATTCTATATACAGTTTCATTTTTTGATATAGTTCTGTAG	3	0.2638522427440633	No Hit
TAAAACAACCAGATAGTTTCGCTTTGGATAATGTCGGAGCGAAGCAAACT	3	0.2638522427440633	No Hit
AGCAGATGGCCATGCTTCGAGCCATGCAACAAGGTGGTAATCAAATGAAC	3	0.2638522427440633	No Hit
ACTGGATCCATCTTGTCACCCAACTACACGGAAGCTGCATCCATCACCTG	2	0.1759014951627089	No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC	2	0.1759014951627089	No Hit
GATAGATAGGACTGTATAGAAGACATTTCGGATGAAATGTGTCCATTCAT	2	0.1759014951627089	No Hit
GAAGAAAAAGACCAAAACGGAAGAATCAAAAGCTGAAGAAACGTCCGAGG	2	0.1759014951627089	No Hit
GGATTTGGCAGTGGTGGATCTGGCAGCAGTGGATCTGGCAGTGGTGGATT	2	0.1759014951627089	No Hit
GATCGGAAGAGCGTCGGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	2	0.1759014951627089	Illumina Single End PCR Primer 1 (100% over 34bp)
TTTTTATCTATTCTGTTGTGTTTTTGTTGGAAGTTAAAAAGCTTTGTTTC	2	0.1759014951627089	No Hit
ATTTTACTTAAATCTGGACTCATGTCATGAAATAAAAAGTTATAGAGGAA	2	0.1759014951627089	No Hit
CCTGTGTCTACTTGTCCTTTTGCCTTTGGTGTACTGTGCCAGTATTGATG	2	0.1759014951627089	No Hit
GTGACACATACAGAAATAAACTGCTGTAGATAAAAAAATATATAATACTC	2	0.1759014951627089	No Hit
ATCGAAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	2	0.1759014951627089	Illumina Single End PCR Primer 1 (98% over 50bp)
TGGATACCCAGCCCAGCCAGGATACCCACAGCAGCCTCCATATGGCCAGC	2	0.1759014951627089	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	15	8.488007E-5	147.61905	1
GCCGTTC	10	0.005800883	147.61905	1
CGTTCCT	10	0.0067135636	140.90909	3
GTTCCTG	10	0.0067135636	140.90909	4
CTGGGCG	10	0.0067135636	140.90909	8
CCGTTCC	10	0.0067135636	140.90909	2
TTCCTGG	10	0.0067135636	140.90909	5
TGGGCGG	10	0.0067135636	140.90909	9
TCCTGGG	10	0.0067135636	140.90909	6
CCTGGGC	10	0.0067135636	140.90909	7
GAGCGTC	20	3.2610455E-4	105.681816	9
AAGAGCG	20	3.2610455E-4	105.681816	7
GAAGAGC	20	3.2610455E-4	105.681816	6
TCGGAAG	20	3.2610455E-4	105.681816	3
CGGAAGA	20	3.2610455E-4	105.681816	4
AGAGCGT	20	3.2610455E-4	105.681816	8
ATCGGAA	20	3.2610455E-4	105.681816	2
GGAAGAG	20	3.2610455E-4	105.681816	5
GTAGATC	20	0.0064074066	28.181818	30-34
GAGTGTA	20	0.0064074066	28.181818	25-29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content