FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-gonad-RNA-1_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-1_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1018.883025505716798TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC494.309586631486368TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC201.759014951627089No Hit
GAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAA110.9674582233948988RNA PCR Primer, Index 40 (96% over 29bp)
CTTAGGAGAAATAAACTCCCCCACAATTGGCATAAAAGAATGATTGGCCC110.9674582233948988No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC110.9674582233948988No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT110.9674582233948988TruSeq Adapter, Index 6 (100% over 50bp)
GCACACCCATGAGAAATCAAGTGTGCACCCATGAGAAACCAAGTGCACAC100.8795074758135445No Hit
TACTCTCAAACACTCCTTCTTCAGACTTTCCCTCGTACTGCTCCAAGCGC90.79155672823219No Hit
AAGAACTATCCTTCTTTAGATTTATTTCTAAAAATAAACATTAATGAAAT90.79155672823219No Hit
TTACTGTTTAAGTCAGCAGTACTGGATAGAGTTATTTACAAAACATCTCA70.6156552330694811No Hit
GAATGGCGTCATAATTTAGTATGCGGTAAATGGCTATGAATGGCATAGAA60.5277044854881267No Hit
GTTTAAACAATCATCTGATTCTCCCATTCTTCTTATGGCCCCACAGATGG60.5277044854881267No Hit
CTAAAACAGAACCTTGAGGAACTCCAGCAGATGTTACCTGACTGTTGGAC60.5277044854881267No Hit
TCATCATCTGACATAATCTGGAAAAGTGACTCATTGGTTCTCTGACAGTA60.5277044854881267No Hit
AAAACCTGACAAAGATGCCACAAAAAAAGAAAACTACAGGCCAATATCAC50.43975373790677225No Hit
CGGCGAGAGTATAATCAAATTCAACACAGCTGCGAAAGGAAACAGTGTGT50.43975373790677225No Hit
AGACTGTTTACATGGAAAAAAATCTCCGTCCAGACTGGTTTAGAAATGCC40.3518029903254178No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.3518029903254178No Hit
TATTTATTTTCGCATAACAAATAGTACATAGTTCTTTTATGTGTAACGTT40.3518029903254178No Hit
AGGTCGACGTTTAGGGCACCGTCAAATCTAAGAGAGGCGGTGATAGATGA30.2638522427440633No Hit
GTGGCTGGGGCTGGGGCTGGGGCCTTGGCTGGGGCTGAGATTGAGGCTGG30.2638522427440633No Hit
CACAATCACCGGTAAATGTATATAAAGCAGAAACGTTTATTTCTTCATCA30.2638522427440633No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2638522427440633No Hit
GATCGGAAGACACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC30.2638522427440633TruSeq Adapter, Index 6 (100% over 40bp)
CTATCTTATTTCCTCAGCTGGGTACTGGCTGTTTATCCAAATGTGTCCAG30.2638522427440633No Hit
TTTGGTTACTGTGATCTTGTGGAAGCCGTTGACGCCCATCAACTGTTTAG30.2638522427440633No Hit
TTTTGATTGACAAAATGAAACTGTATACAGAATAACTAGTATTAGTAAAT30.2638522427440633No Hit
CCCAGCTTCACTTTTAAACATCTCAACTTGAGATTTACCTCAACTCATTT30.2638522427440633No Hit
CAACTCCTCAACATACATTGTAATTAAATAAATAAATAGAATTGCTTGTA30.2638522427440633No Hit
CCCGTATCAACCTTTACAACGACCATCCCGCTGAACTCATTCTCGAACTC20.1759014951627089No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1759014951627089No Hit
CCCCACCCCACATGTTCAAACATGTTTGATAATTAAGTAAAATAGTAACA20.1759014951627089No Hit
GGTTGAGGAAACATCCTCAAAAGTTTATTTGCCTGTTTTTGGTTCACTCT20.1759014951627089No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1759014951627089No Hit
ATCTAGTAATACATTTAACATACACGAGTGTGATCAAAACGTCTTGTTTA20.1759014951627089No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC20.1759014951627089No Hit
AATAATAAATAGGTTTAAACTAAACGAGTGCCTACTTGGTGAAATAAAAA20.1759014951627089No Hit
CACTTTACTAACTCAATACTAATACATGTACTGAAAAACAAAATAGATTC20.1759014951627089No Hit
CTCTGTGTCACCCGTGCTTGCCAGGGGACCCCCTGAGGTCCTTGCTCTTG20.1759014951627089No Hit
ATATACTAAAATAACATTCAACATGAGAAAATTTGGCTGCAGTGATTAGC20.1759014951627089No Hit
TTTTGATGGTCATTTAAACAGGCTGAACTCCAGTCACGTTTCGGAATCTC20.1759014951627089TruSeq Adapter, Index 21 (95% over 24bp)
TGGATATCACATATATGCAATAAATTTGTGAATAACATGGAATATATTGT20.1759014951627089No Hit
CTCGCCAACAGTCTCCGTGATTATTGATATACGGCATTGTATCTCATTAT20.1759014951627089No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1759014951627089No Hit
GATCGGAAGAGCACACGTCTTAACTCCAGTCACGCCAATATCTCGTATGC20.1759014951627089TruSeq Adapter, Index 6 (98% over 50bp)
TGGAAAACATAAAACACCGGGTATACCAGCAACCACACACATGATGCTGT20.1759014951627089No Hit
GCCAAATCCACCGCTGCCAGATCCACCGCTGCCAAATCCACCACTGCCAG20.1759014951627089No Hit
AGCGGGGCAGCCACCTTTGGGTTTGGCGAATGAGTACGTTCCTTTGGGCC20.1759014951627089No Hit
TTTGAGACCTCCGAATTGGAACGTGATCTTATCTCATTGTCGGAAAAATC20.1759014951627089No Hit
CTCGTAGTATGTGGATGTCACAATGTCAGCCTGACAAAACTGACACTTGA20.1759014951627089No Hit
TTTTAAGTAGATAAAATAAATGAGTGATTGTTTATTTATAAAGACAATCA20.1759014951627089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA158.6873755E-5146.76191
CTTAGGA100.0059028147146.76191
GGAGAAA100.0068315133140.090915
GAGAAAT100.0068315133140.090916
TAGGAGA100.0068315133140.090913
TTAGGAG100.0068315133140.090912
AGGAGAA100.0068315133140.090914
AAGAGCA203.3375353E-4105.0681847
GAAGAGC203.3375353E-4105.0681846
TCGGAAG203.3375353E-4105.0681843
GAGCACA203.3375353E-4105.0681849
CGGAAGA203.3375353E-4105.0681844
AGAGCAC203.3375353E-4105.0681848
ATCGGAA203.3375353E-4105.0681842
GGAAGAG203.3375353E-4105.0681845
ACACGTC200.006592373428.01818310-14
GTCACGC200.006592373428.01818325-29
ACGCCAA200.006592373428.01818330-34
CAGTCAC255.537039E-428.01818325-29
CACACGT200.006592373428.01818310-14
Produced by FastQC (version 0.11.5)