FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-8_S59_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-8_S59_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2034.4226579520697165Illumina Single End PCR Primer 1 (100% over 50bp)
TTTGTAATGACGGCTATCAGACAAGTAACGGAGCATGTACAAACATTGAC420.9150326797385622No Hit
GGCAAGTGTTTTGTAATAAGGAAGTTACATACTCATTTTATTTTTATATA370.8061002178649237No Hit
GTGAAAGAGAACAACCGGTGCTGTTACAGTGTCCAGATGGCAAAGATATA340.7407407407407408No Hit
TGTGAATTTCATTTTTTTGTGCTGTTTGTATCAGTCCTATCCATATATGA330.7189542483660131No Hit
GGGAATTATGTTTATATTAACTGACAGAGTGGAAAACAGTGCAGGAAGTG310.6753812636165577No Hit
GTTGACCTCCAGTAGGGGTTTTGCCCATTAATGCTTTTGGGGTGCCGTTG300.6535947712418301No Hit
GGAGAATTGGCATCTCACTCTCGTGCATTGCTACCCCACCCTGAACTGTC290.6318082788671024No Hit
GGTGGCAGTTATGTGATCACACAGAAATGGATCCATCAGTTTGATGTCAT290.6318082788671024No Hit
TGGAACTCCAACCGCTATAGCTAAAATAAAACAAGACACTCAGAAAGCAC290.6318082788671024No Hit
TTCACACAATCCTTCCCAGTCATCAAGCCTGCTTCATAAATTAGATAACT260.5664488017429194No Hit
GGGACACACAGTGATTTTGCTAAATTTTCACTAAACACAGCCACTGTTTT260.5664488017429194No Hit
CGCGATAGTGAAATAGAAATGGACGATTCGGCGTCCGTACCGGTCATAAA260.5664488017429194No Hit
GTCATTTTTGTGCCATCGACAGACGGTCAGTTAACCTAACCACTGTTTCC250.5446623093681917No Hit
TGTATTTGCTGAAGTATCAAGATGAAGTTCTCAATCGGTTTACTGTTTGT250.5446623093681917No Hit
AAACCAGAACTCAGAAAAAAAGTTATGCCTGCTGTGGTAGTGCGGCAGAG250.5446623093681917No Hit
TTTGATCTCCCTAGTGGAGATATTTTGTTACTCACCTTTGGTTTAAACAT250.5446623093681917No Hit
GAGTGAACTATGAGGGAGTCAATCCGAGTCTGAACTAGTTACATGCACAC240.522875816993464No Hit
TAATATACAGCTTCAAAAGTTAGCGGAAATTGAAGAAGAGAATGAGTTAC240.522875816993464No Hit
TTTGCATTTGATATAAATGTCATTTTTCAGTATACATCAATATTAACATG240.522875816993464No Hit
TGAAAACGCCTCTTTTAATACACATTCAAATGGTAAGAGTGACTATAGTT230.5010893246187363No Hit
CCACAAATAATGAAGACGATAGTAATACGGCATCACCTGGTCCACAAACA230.5010893246187363No Hit
CAGTGTATTAAGTTAAGTTACACATTATTAAATCCAGTTTGGACTATTGT230.5010893246187363No Hit
GTTGGAATTATTTGGACATTGTATATAAAGCTGTCAACAATACAATTTGT230.5010893246187363No Hit
AAAACTAAGAAAATAGAAGGAAACTAAGAAAATTAGAAGGAAACTAAGAA230.5010893246187363No Hit
GAACGTTTGTTTGTTCTTTATTTTGCATTTTCTTGTACAGATCTAAACAT230.5010893246187363No Hit
TATTGGGGTAGTGTTAAGGATTAGTGTTTTTGTGAGAAGAAAAACGGTAA230.5010893246187363No Hit
CGAGACTAAGCAAAGTACCAAATCACGTTGGATAATTGGAATAATCATTG220.4793028322440087No Hit
AGGATCTGTACGCAAACACCGTATTGTCCGGCGGTTCGACCATGTACCCA220.4793028322440087No Hit
TGTCATTCCATACAAAATGTCGTACCAGACAATACAGCCAACATCATACC220.4793028322440087No Hit
TGAAAACCCTGTTTGGTATTGGGATGCTTCGTGTATGTGTATGATTTGCA220.4793028322440087No Hit
GAGCAACAGTCGCTTTCGCATCGGGTCAATTAACAAGTTTATCACAAACA220.4793028322440087No Hit
GGAAACGACACCGTCTTGCTCTTAAGAGACGTCGTTCCAGTAAGAAACGT220.4793028322440087No Hit
GGGGAGCTTACTTTGTTCCAGGTGTATATAAAACAATATAATTATTATGC210.4575163398692811No Hit
GTATAGACTGTTGGCTAATCACGTCAGAGAGAGCGGGTTCGATCCAAGCG210.4575163398692811No Hit
CTTCCATAAAGCGATATCAATGGTGATACCACGTTCTCTCTCAGCCTTCA210.4575163398692811No Hit
CCTCAAATTCACTGTTTTATTATTGTGTTAAAAGTATATATATATGAACA210.4575163398692811No Hit
AAATATTAAACTTTATTGGTAGTTAATGAAACTAATCTTCATCAAGGAAT210.4575163398692811No Hit
CGAAGATCCACATTTAAAATATTTTAAACATTGCCTGGTGGTCTGTAAAG210.4575163398692811No Hit
TCATTATAAAATGCAAGGCAAAAAGATAACAAATGACCATATCACTTCTT210.4575163398692811No Hit
AAGATCAAGTTCCCACTGACCCACAGAATCCAAAGGAAACTTCACGCTCC200.4357298474945534No Hit
CAGGAAGCCACAGAGATGTTGGTAGGCAACGCGCAAAATCTCATGCAGGC200.4357298474945534No Hit
GTGGTTTCAATGGATAGATCATCACATCTTCTTTTGTCATAAAACAATAA200.4357298474945534No Hit
ACAATATGTACATGAACATGCATTTATAATCAAATTACTTTTTTTCTTTG200.4357298474945534No Hit
GTATGAGATATATAGTAAATCGATGTTGCACACAGATTCACTATTAAATA200.4357298474945534No Hit
TTCTGATTTACATCTGTAGACAAAGGGATGACTGCTCCAATAATGGTGTT200.4357298474945534No Hit
CTATAGTTAATAACCCGATGCCATCTCGGAGTGTGTCACGTTTTTCATCT200.4357298474945534No Hit
AAGAACAACAGCAGCACGAGGAGCCGGAGACAAATGAAAACCCTGACACC200.4357298474945534No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT200.4357298474945534Illumina Single End PCR Primer 1 (100% over 50bp)
CCCTGCTCTGTCACAATCTCTGTGATTGTTGATATACAACAATGCATCTC190.41394335511982566No Hit
TGAAAATGACAAGTTGTTTTGATGTGTATTTGTAAAAACAAAACAAAGTA190.41394335511982566No Hit
TTTTGTTTTACACTGATGTTACAAGGTAAAATGGACCATTGTTCTGTTTT190.41394335511982566No Hit
GGTTTTTAGGTGTTTTATGAATTCGAAATATTTCTCAGAGAATGAATTAA190.41394335511982566No Hit
TGAATGTTTGGTGAGATGTTTACCTATATGTCATGAATGTTTGGTGAGAT180.39215686274509803No Hit
AGTATTTCCACTGTATAATTATTGCAAAATCGGAAGAAGTATTGTAATAT180.39215686274509803No Hit
TGTAATGAACCACGGACTTAAAGAATGTGACCTGTTTCTCACTAAGAAAA180.39215686274509803No Hit
AAAAAATGCAATTACTTATAAGTTGGCTGTTTCAATATTTTGAAGTGGAA180.39215686274509803No Hit
GTTAAGTTGTACATGAAGTTTGTTACATGTAGTTTGATACATGTAGTTTG180.39215686274509803No Hit
TTCCATTCCATTTCTGAAATTTCACATCTAACAGAAATGTTGAAAAAAGT180.39215686274509803No Hit
AGATTCAATACCAGTGCATTTGCATATACTTAATATAATGATATATACCA180.39215686274509803No Hit
TTTGCCATCTGTGGGGCCATAAGAAGAATGGGAGAATCAGATGATTGTTT170.3703703703703704No Hit
TGGAGTCTGGTTGAGATACGTGTCCAGAAGTGGTACACACAATATGTACC170.3703703703703704No Hit
GCCTTCCCTCACATTCAGGCCTTTCAGGCCTTTGATTTTATTTCTTACCA170.3703703703703704No Hit
TCTGTGCCTGCTGGCTGCAGCGGGCATTGTTGCCAAGGTTACATGCTTGA170.3703703703703704No Hit
GAATTGAGGTTGAAAGGTCACCAGAAGGAAGGATATGGTCTGTCGTGGAA170.3703703703703704No Hit
GGTAGGGTAGATACAGCCTTTAACAGATTTATTTTGCAACATTGTATTTT170.3703703703703704No Hit
CTCGCTTCTGAAACTTTGAATAAATACAATTCGCTGCTGAACTTTGAATA170.3703703703703704No Hit
CATACAGTGCCTTGCAAGGTAACTGGAAAGTGTGGTAGTGTAACTGTACG170.3703703703703704No Hit
TTCAGATCTCTTTAGTGTATATAAATATCCCTAAACCAACTATGGATCAT160.34858387799564267No Hit
CGGCCATTCACAGGGCTCAAAGGCCAAGCCGCCCCGACAAGGCCAGACGT160.34858387799564267No Hit
TTTGAACCTACCAGAGCTGGGACAACTACCTGCACTGAATTTGGAAGATG160.34858387799564267No Hit
AATTGAGGATCGTCATGACAAACCTGGGCGAGAAACTTACAGATAAAGAA160.34858387799564267No Hit
ATCACCATGCCGTCAAACCTGTGTAAGCAGTCACCTCTATTAAGCAGCCA160.34858387799564267No Hit
TAAGTAGCCGGTACAGTGTCAAACACTCACTTGGGGAGCATCCATCTGTT160.34858387799564267No Hit
GGAATATTGCGAGTCGGTGGACTACAGAGGCAGACCTATTGGAGAATATG150.32679738562091504No Hit
TGCGTATCTATGGAGTAAAGGGTTAAAAAGGTTACCAACAACAAAATATT150.32679738562091504No Hit
GGAGACCAGAAAATAAGCATGATATCAACTAGAACAAAATTATGTATTTT150.32679738562091504No Hit
TTAAAGTCCGAGTTACATCACAAGGCGACCAGACTAAGGTTTGGATTAAT150.32679738562091504No Hit
CGAAATCGTTCACATACAAGCCGGACAGTGCGGAAACCAGATCGGTGCAA150.32679738562091504No Hit
CTATTGTAAACAATTCTAAAATTCATTGCACACTGCGGTTCATATTGCCT150.32679738562091504No Hit
CACTCCGACTGCGCCTTCATGGTCGACAACGAGGCCATCTACGACATCTG140.30501089324618735No Hit
AACCATTGAGGAGGTTGATTAATTAAGTGTATGGTTAACTGAACATTAGA140.30501089324618735No Hit
TTTTTTAAATGTTGTGTGCAAATAAACTGATATTTTTCACATCACTGGTA140.30501089324618735No Hit
GGCTACCAGAAGCGATAGGCAGGTCCTGTCTGAGCGTGGGTCTCTTTTCA140.30501089324618735No Hit
GCGGTTTCGAGCTAGCAATTACCAGAGTACGACTCGAGTTAAGCCATTCA140.30501089324618735No Hit
AACATAATATACGTAGATATGATATCGTGCGTTTCACAACTGATCCCAAT140.30501089324618735No Hit
GTTTTACCCATCTGGCCCTGCTCTTTCCAGCTGGTCATTATGCCAATCTT140.30501089324618735No Hit
GTTGAGCATGGTTTAAATTAGACGTGAAAAATCAGTTTGCTTAACTTTAA140.30501089324618735No Hit
GTTGAAGTTCATTTTCTTATATCTTTATTATCTGTGACAAAAGGTGTCAA140.30501089324618735No Hit
CTTAAAAAGAATCCTCTCAAGAATAAGCGTGTTATGGACCGTCTAAATCC140.30501089324618735No Hit
GTTGGTAACTCACATCAGAGGGTTTGATCCCCAGCTCAATCCGATGCCAA130.2832244008714597No Hit
GACAAGATACGCATCTGAACCAGAGAACCCCACAAAATCGGCCAAGGCCA130.2832244008714597No Hit
GAATGTTCCATAACAAGTTGTTGATGAATCAGGAATTAGGGAGATCAAAG130.2832244008714597No Hit
CAAGATCTGGGATCTTGAAGGCAAGGTTGTAGTGGATGAGCTGAGACAGG130.2832244008714597No Hit
AAAAAAGCAAACAAACCAACATTGATTGCCAAGTCCAGTTTACTTATTGA130.2832244008714597No Hit
TTCAGGGCCCTGTAGACCCATTCAGACAGACGATGAAGACAAAGTCAAGA130.2832244008714597No Hit
CCAGCTCAACGATACAATTTCATTGACAGTCAAAATCAATATCTTCCAGA130.2832244008714597No Hit
CATGCGCTGACGGTGAAAGTGTAACAAATTAATACTATTTATCTTTGTCT130.2832244008714597No Hit
CAAAACTGAATTTCACTTTTTATTAGTTTGACAAAGATTTAATTTAATTC130.2832244008714597No Hit
TGAACAGACTGATTGCTCAGATCGTATCCTCCATCACTGCCTCTCTCAGA130.2832244008714597No Hit
TTGCAGAATACCACCAAATGAATATGGACAACCGAAGGGATTTGCGTTTG130.2832244008714597No Hit
CTGACATTACAGACCCATGTGAAGAGTGTGTAATATGTATACACGTGTAT120.261437908496732No Hit
TTGATATTTGTCAATGTCGGTCAAATCTTGCAGGAGTTATGGCTCCTTTT120.261437908496732No Hit
GTAGAGGCTGATATGGAATACCTGGAAGAAAATATTCCAGCAGCATCTTC120.261437908496732No Hit
GGGTGTCGTAAATTGTACTGAAAATGGGGTTGATGTTTTTTTTCCTGGTG120.261437908496732No Hit
TGGGGACGCGTCAGAAAAAAAAGATATGTCGTGGGTGTGTCACAAGTCAG120.261437908496732No Hit
TCCAGACCAACTTGGTACCATACCCACGTATCCATTTCCCTCTGGCCACA120.261437908496732No Hit
GAAAAGCATATTAACACAAATTCATCGTGCACAACGTCAACGAGTAGCAG110.23965141612200436No Hit
AACAGAATGGCGTCACCAAAATCCGTTTTTTCAAATGATATGTTTACAAT110.23965141612200436No Hit
GGATGCCTGGTTTGTTACGTTCATGACTTTGGTGTTGATGTCATGCCTTT110.23965141612200436No Hit
ATGCACCCTGTTTGAAGGAAGGGGCAATTTTTTTTAATGTTCTTAGGAGT110.23965141612200436No Hit
TTAAATGATAGCTTAAATCAGATTTTTAACAAATGTATATTTGTTATCAT100.2178649237472767No Hit
GTCAGAATCCCGCCCAGAATGCCGTAACGATACTCTGTGCACCGCCGTCG100.2178649237472767No Hit
GTTAAAGCGAGTTAGCTTATTTGTATGGTCAGTTGCTATCACTGCTTTTT100.2178649237472767No Hit
TGGGGGGATTCAGGATTCCCGTTTTTTTAGCGGGTTGTGGTTTAAGCTCC100.2178649237472767No Hit
TCTCATTGTAAGGTTTTACTTCTGTGGGTAATAGTTGAGACTTATCTCAT100.2178649237472767No Hit
GCTTCCTAGAGGTATACTATGGCGGATTAAACCAGTTGAGTTATTTATAT100.2178649237472767No Hit
CTCCAGTGTGGCAGCGTTCAGAATACTGTCTCAAGTAAACACTGGCGCGA100.2178649237472767No Hit
TACCAACTTCCAACTGAAACAGAACCAGCCCACAATCCCGGAGCCAGACT100.2178649237472767No Hit
ATGGCCTGCACTTTTTCCCCTGAAAAATAAAAAATATATATAATTTAATT100.2178649237472767No Hit
TGACCTTTTCCTTCACTGTCTAAAACCTGAAATGCTCGAAGAATCTGATC90.19607843137254902No Hit
TAAAAGACGGGCAATCTTGTATCGTTGTCGGAACAGAATCTGATTGACTG90.19607843137254902No Hit
AACATGCCAAAATATATTTTTAATAGAGTGGTAAAATTCAAACGAAATCC90.19607843137254902No Hit
CTTGTACATCATAATTGCAGTGGATACCAGACTTAGAGACCCCTGCTGTT90.19607843137254902No Hit
TCCACGAATTCCTGTATGGCTAGGAAGCCAGCAGAAAACAATAGTATTAT90.19607843137254902No Hit
GGAAAATGTAAAACACTACCAAAATACTATGTGACAAAGGTTGCCGATTA90.19607843137254902No Hit
TGGTGATGATGATTTTATCTTCAGGAACGAATAATTGCTGCATAATGTGC80.17429193899782133No Hit
CAAGGATGAAGATGAACAGCAAAAGGATCGTATCCAATCCAAGAATGGTT80.17429193899782133No Hit
TATTGGTTTTATTGTATTTTCAACTGTTGTTAAATTTGCTTTTTTTTATC80.17429193899782133No Hit
TCATGCGAATGACTTTGGTCTGTTCTTCAAAAATCAAGGTCACATATCAG80.17429193899782133No Hit
CTTTAAAAAACTTTTTTTTTTATTTTTTCATTTTAAGTTTCCGTCGGGAC80.17429193899782133No Hit
TTTAAAATGACTAGTAAAAATCAAAATCTGGCAAAATAGATAAGCTTCCT80.17429193899782133No Hit
CAAACACGGACTTTGCTATCAGTACCACAGGAGTAATAACTGTCGCAAAG70.15250544662309368No Hit
TTGAGAAACTGTGCAGTGATTATCTCCCTTTTTCAGAGCGACCAGAATCG70.15250544662309368No Hit
AAGTTTGGATGACAGTGGGTTCTGTTCGATGAAGCTATAACAGTTTAAAG70.15250544662309368No Hit
CGTGTTTCTATGGGGATTCTATGCATAACGCGCAACTAGTACAACTTACA70.15250544662309368No Hit
TTTAACTTCTTTCATTTGGGACTCTCTATGAATGACGAACAGTCACATTT70.15250544662309368No Hit
AACAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTCAGACGTGT60.130718954248366TruSeq Adapter, Index 12 (100% over 51bp)
GTATTGAAACAAAGGAAGACAGATTTGCTTTACTTGGTGGCTTGAGTAAG60.130718954248366No Hit
CCTCAAATTCACTGTTTTATTATTGTGTTAAAAGTATATATATGAACAGA60.130718954248366No Hit
AAACAATATATTTTTAAGGATGGCCATGTAAAACTGGTGGAAAATAAATA60.130718954248366No Hit
TGATCCTAAAGAGATTTACAACGAGGCCCAGGAACTAGAAGAATGAATGT60.130718954248366No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10893246187363835No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.10893246187363835No Hit
CTGTTTTATGTTTGTACATTTAAGAATTGATTTTCAATTTTCAGGTTGGA50.10893246187363835No Hit
ACAAGTGTTCCTTATTCACAAAGGAACTTGCTATGTACAATATATTAAGC50.10893246187363835No Hit
GGGAGTTTCATCCTCTAACTTCCCTGAAACCAAATTTACAAATGTGATCA50.10893246187363835No Hit
CCGCATACCAGTGATGAGAAAACATTTATGTTTACACTCTTACATCAACT50.10893246187363835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTTT157.264255E-5162.32051130-134
GCCTTCC100.007509218140.677781
GCGGTTT100.007509218140.677781
CTCGCTT100.007509218140.677781
CGCGATA100.007509218140.677781
GAGCGTC202.3444609E-6139.131879
GGTTGAA100.0077642947139.131878
CGATAGT100.0077642947139.131873
TAGTGAA100.0077642947139.131876
TGACCTC151.3577437E-4139.131873
CCAGTAG151.3577437E-4139.131879
GCGATAG100.0077642947139.131872
CGCTTCT100.0077642947139.131873
TTCGAGC100.0077642947139.131876
CTCCAGT151.3577437E-4139.131877
GTTTCGA100.0077642947139.131874
GAGCTAG100.0077642947139.131879
GGTTTCG100.0077642947139.131873
TCCAGTA151.3577437E-4139.131878
TCGGAAG202.3444609E-6139.131873
Produced by FastQC (version 0.11.5)